胆囊癌中ER、PR、p53 表达及DNA 含量的研究

 目的 探讨ER、PR、p53表达及DNA倍性在胆囊癌发生发展中的意义。方法 用免疫组化二步法及HPIAS-1000DAN图像分析系统检测27例胆囊癌组织中ER、PR、p53的表达及DNA倍性。结果 ①胆囊癌中ER、PR及p53的阳性表达率分别为77．78％(21／27)、74．07％(20／27)、66．67％(18／27)，②胆囊高分化腺癌中DNA倍体为高二倍体；中分化腺癌为高二倍体至亚四倍体；低分化腺癌为四倍体。③胆囊癌分化越差，浸润越深，其ER、PR、p53的阳性表达率越高(P<0．05)，DNA含量越高，多倍体越明显(P<0．05)，预后越差。结论 ER、PR、p53阳性表达率及DNA倍性改变与胆囊癌的分化程度和浸润深度有关。ER、PR过度表达可能会诱发p53基因突变，两者在胆囊癌的发生发展中有密切的内在联系。

Expression of ER、PR、p53 and DNA Content in Gallbladder Carcinoma

Objective 　To investigate biological significance and relationship of ER、PR、p53 gene protein and DNA content in occurrence and development of gallbladder carcinoma. Methods 　The two steps of immunohistochemist ry and HPIAS-1000 DNA image inst rument were used to determine the expression of ER、PR、p53 and DNA content in 27 cases of gallbladder carcinoma. Results 　① In 27 cases of gallbladder carcinoma , the positive expression rates of the ER , PR , p53 were 77. 78 %、74. 07 % and 66. 67 % , respectively. ② The DNA ploid in high , middle and low differentiation gallbladder carcinoma were over doubleploid , ranging f rom over doubleploid to sub2quardriploid and sub2quardriploid , respectively. ③ In gallbladder carcinoma , with the increasing of the invasive depth and the lowering degree of differentiation , the positive expression rate of ER , PR and p53 were rising up , so was the DNA content s. Conclusion 　The positive expression ER、PR、p53 and DNA changeful ploidesis is related to the level of differentiate extent and infilt rated depth of gallbladder carcinoma. Excessive expression of ER、PR will possibly induce the mutein of p53 gene. They have closely inside relationship in the occurrence and development of gallbladder carcinoma .