一氧化氮和Ca~(2+)含量对缺血再灌注大鼠脑细胞凋亡的影响

目的:通过观察脑缺血再灌注时脑组织一氧化氮(NO)和Ca2+含量及脑细胞凋亡的变化,探讨NO、钙对脑细胞凋亡的作用。方法:本实验在建立大鼠急性全脑缺血再灌注损伤模型的基础上,用Green法测定脑组织NO的含量,用原子分光光度仪测定脑组织Ca2+含量,用流式细胞仪检测脑凋亡细胞。结果:脑缺血再灌注12h、24h,NO含量及Ca2+含量均较假手术组增加(P<0.05,P<0.01),脑细胞凋亡数亦增加(P<0.01),且随时间延长进一步增加(P<0.05)。脑缺血再灌注12h、24h时,脑组织Ca2+含量与脑细胞凋亡数呈正相关(P<0.05)。结论:全脑缺血再灌注损伤时发生脑细胞凋亡与脑组织NO、Ca2+含量增高有关。

Influence of NO and Ca2+ Concentration on Cerebral Cell Apoptosis after Cerebral Ischemia Reperfusion

Objective: To investigate the role of NO and calcium on cerebral cell apoptosis after ischemia reperfusion.Method: Use Pulsinelli's methods to create rat model with cerebral ischemia reperfusion injury.The production of NO was measured by Green methods. Atomic absorption spectrophotometer(AAS) was used to analyse calcium concentration,and flow cytomyter(FCM) was used to analyse apoptosis.Results: The production of NO and calcium concentration during ischmia reperfusion 12,24 hours are higher than sham-operation group(P<0.05,P<0.01). At the same time apoptotic cerebral cells number increased with time-dependent manner.Variation of calcium concentration and apoptotic cerebral cells number show a marked positive relationship during ischemia reperfusion 12,24 hours(P<0.05).Conclusion: The cerebral cell apoptosis after ischemia reperfusion relates to the increasing of NO and calcium concentration of brain tissues.