抑癌基因p53及p16单独或共转染治疗非小细胞肺癌的动 …

目的 观察抑癌基因ｐ５３和ｐ１６单独或或共转染在动物整体中治疗非小细胞肺癌（ＮＳＣＬＣ）的效果。方法 采用培养细胞移植法，将２ ＮＳＣＬＣ细胞系Ａ５６９接种于裸小鼠背部皮下，建立裸小鼠皮下肺癌移植瘤模型。将２５只荷瘤裸小鼠随机分成空白对照组、十八酰基胺阳离子（ＳＡ０对照组、ｐ５３基因组、ｐ１６基因组、ｐ５３＋ｐ１６基因组，共５组，每组５只。采用瘤体内直接注射的方法，用ＳＡ脂质体介异，将ｐ５３和（或

Gene therapy with tumor suppressor gene p53 and(or) p16 on the nude mice models of NSCLC in vivo

Objective To investigate the effect of tumor suppressor gene p53 and(or) p16 treatment on the nude mice models of non small cell lung cancer (NSCLC) Methods Nude mice were injected subcutaneously with NSCLC cell line A549 25 nude mice were randomly divided into 5 groups (control, SA, p53 gene, p16 gene, p53 p16 genes), p53 and(or) p16 genes mediated by stearylamine/DOPE (SA liposome) were injected intratumorally alone or jointly The size of tumor and survival period of nude mice were measured after treatment Results p53 and(or) p16 genes can obviously inhibit the growth of tumor The difference was significant between control and treated groups, among which the combined p53 and p16 genes enhance the inhibiting effect more markedly The survival period of tumor bearing nude mice was prolonged after transfecting p53 gene or p16 gene alone, p53 and p16 genes jointly can prolong the survival period significantly Conclusions Tumor suppressor gene p53 and(or) p16 in the replacement therapy of NSCLC are of potential clinical significance The combination of p53 and p16 gene may have a greater antitumoral effect in vivo