| 线粒体DNA与人精子活力间的相关性分析 |
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| 引用本文： | 郑英,周作民,沙家豪. 线粒体DNA与人精子活力间的相关性分析[J]. 解剖学报, 2000, 31(1): 34-38 |
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| 作者姓名： | 郑英 周作民 沙家豪 |
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| 作者单位： | 1. 扬州大学医学院组织学胚胎学教研室,扬州,225001
2. 南京医科大学江苏省生殖医学重点实验室,南京,210029 |
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| 摘 要： | 目的 探讨线粒体ＤＮＡ与人精子活力间的关系。方法 用长链ＰＣＲ技术，对６０例精子活力正常和４０例精子活力异常不育患者的精子线粒体ＤＮＡ（ｍｔＤＮＡ）进行了多重缺失的分析。结果 两组不育患者中共有８例具有ｍｔＤＮＡ的多重缺失（其中精子活力正常不育患者６名，精子活力异常不育患者２名），但缺失型ｍｔＤＮＡ（Ｓ５除外）在总ｍｔＤＮＡ中所占比例很小（０．１６％～１．８５％），１例精子活力正常的不育患者（Ｓ５
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| 关 键 词： | 线粒体 DNA 精子活力 长链 聚合酶链反应 |
| 文章编号： | 0529-1356(2000)01-34 |
| 修稿时间： | 1998-07-17 |
ANALYSIS OF THE CORRELATION BETWEEN MITOCHONDRIAL DNA AND HUMAN SPERM MOBILITY |
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| ZHENG Ying,ZHOU Zuo-min,SHA Jia-hao. ANALYSIS OF THE CORRELATION BETWEEN MITOCHONDRIAL DNA AND HUMAN SPERM MOBILITY[J]. Acta Anatomica Sinica, 2000, 31(1): 34-38 |
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| Authors: | ZHENG Ying ZHOU Zuo-min SHA Jia-hao |
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| Affiliation: | ZHENG Ying;(Department of Histolog and Embryology,Medical College of Yangzhou University,Yangzhou225001 |
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| Abstract: | Objective To analyze the correlation between mitochondrial DNA and human sperm mobility. Methods By using long polymerase chain reaction techniques, we studied multiple deletions of sperm mitochondrial DNA(mtDNA) in 100 infertile patients(60 with normal sperm mobility and 40 asthenospermia). Results Six of 60 patients with normal sperm mobility and two of 40 asthenospermia had multiple mtDNA deletions, but percentage of deletions in mtDNA(DmtDNA) in total mtDNA was very low(from 0 16% to 1 85%).A 2 6kb DmtDNA was found in one of infertile patients (No.S5) with normal sperm mobility, and percentage of 2 6kb DmtDNA in total mtDNA was 91 02%. Sequence analysis revealed that most of mtDNA sequence encoding RNA and polypeptide were deleted. Conclusion There is no relationship between multiple deletions in mtDNA and human sperm mobility. |
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| Keywords: | Mitochondrial DNA Sperm mobility Long polymerase chain reaction |
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