An n-3 PUFA-rich microalgal oil diet protects to a similar extent as a fish oil-rich diet against AOM-induced colonic aberrant crypt foci in F344 rats |
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Authors: | Vincent A. van Beelen Bert Spenkelink Hans Mooibroek Lolke Sijtsma Dirk Bosch Ivonne M.C.M. Rietjens Gerrit M. Alink |
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Affiliation: | 1. Division of Toxicology, Wageningen University and Research Centre, P.O. Box 8000, 6700 EA Wageningen, The Netherlands;2. Agrotechnology and Food Innovations B.V., P.O. Box 17, 6700 AA Wageningen, The Netherlands;3. Plant Research International, P.O. Box 16, 6700 AA Wageningen, The Netherlands |
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Abstract: | The chemopreventive effects of high fat microalgal oil diet on azoxymethane (AOM)-induced colonic aberrant crypt foci (ACF) were studied in male Fischer 344 rats following 8 weeks of dietary treatment. These effects were compared to the effects of high fat fish oil and high fat corn oil diets to determine whether microalgal oil is a good alternative for fish oil regarding protection against colorectal cancer. Despite the difference in fatty acid composition and total amount of n-3 polyunsaturated fatty acids (PUFAs) between microalgal oil and fish oil, both these oils gave the same 50% reduction of AOM-induced ACF when compared to corn oil. To determine whether oxidative stress could play a role in the chemoprevention of colorectal cancer by n-3 PUFAs, feces and caecal content were examined using the TBA assay. The results showed that lipid peroxidation does occur in the gastrointestinal tract. As several lipid peroxidation products of n-3 PUFAs can induce phase II detoxifying enzymes by an EpRE-mediated pathway, the in vivo results suggest that this route may contribute to n-3 PUFA-mediated chemoprevention. All in all, n-3 PUFA-rich oil from microalgae is as good as fish oil regarding chemoprevention in the colon of the rat. |
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Keywords: | ACF, aberrant crypt foci AOM, azoxymethane HFCO, high fat corn oil HFFO, high fat fish oil HFMO, high fat microalgal oil MDA, malondialdehyde PUFA, polyunsaturated fatty acid TBARS, thiobarbituric acid reactive substances |
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