Targeted metabolite analysis of Catharanthus roseus and its biological potential |
| |
Authors: | David M Pereira Federico Ferreres Juliana Oliveira Patrícia Valentão Paula B Andrade Mariana Sottomayor |
| |
Institution: | 1. REQUIMTE/Department of Pharmacognosy, Faculty of Pharmacy, Porto University, R. Aníbal Cunha, 164, 4050-047 Porto, Portugal;2. Research Group on Quality, Safety and Bioactivity of Plant Foods, Department of Food Science and Technology, CEBAS (CSIC), P.O. Box 164, 30100 Campus University, Espinardo, Murcia, Spain;3. IBMC-Instituto de Biologia Molecular e Celular, Universidade do Porto and Departamento de Botânica, Faculdade de Ciências, Universidade do Porto, R. Campo Alegre 823, 4150-180 Porto, Portugal |
| |
Abstract: | Catharanthus roseus is nowadays one of the most studied medicinal plants. In this work, further knowledge on different parts of this species (leaves, stems, seeds and petals) was achieved, namely phenolics by HPLC-DAD and organic acids and amino acids by HPLC-UV. Also, the biological potential, expressed as acetylcholinesterase inhibitory activity was accessed and, in some parts, an acetylcholinesterase inhibitory capacity higher than 85% was found (IC50 at 422, 442 and 2683 μg/mL in leaves, stems and petals, respectively). C. roseus aqueous extract revealed to be a rich source of phenolics, namely caffeoylquinic acids and flavonoids derivatives (up to 4127 mg/kg in stems, 4484 mg/kg in seeds, 8688 mg/kg in leaves and 41125 mg/kg in petals), organic acids (962, 6678, 25972 and 12463 mg/kg in seeds, petals, stems and leaves, respectively), such as citric acid (over 85% in some plant parts), and amino acids (31557, 39327, 50540 and 159697 mg/kg in stems, petals, seeds and leaves, respectively), of which arginine was a major compound. |
| |
Keywords: | Catharanthus roseus Phenolics Organic acids Amino acids Acetylcholinesterase inhibition |
本文献已被 ScienceDirect 等数据库收录! |
|