Inhibitory mechanism of saponins derived from roots of Platycodon grandiflorum on anaphylactic reaction and IgE-mediated allergic response in mast cells |
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Authors: | Eun Hee Han Jin Hee Park Ji Young Kim Young Chul Chung Hye Gwang Jeong |
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Affiliation: | 1. BK21 Project Team, Department of Pharmacy, College of Pharmacy, Chosun University, 375 Seosuk-dong, Gwangju 501-759, South Korea;2. Division of Food Science, International University of Korea, Jinju, South Korea |
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Abstract: | The purpose of this study was to investigate the protective effects of saponins isolated from the root of Platycodi Radix (Changkil saponins: CKS) anti-allergic effects in mice and mast cells. Oral administration of CKS inhibited the dinitrophenyl (DNP)–IgE antibody-induced systemic PCA reaction in mice. CKS reduced the β-hexosaminidase and histamine release from anti-DNP–IgE-sensitized RBL–2H3 cells. In addition, CKS inhibited the IgE antibody-induced increases in IL-4 and TNF-α production and expression in RBL-2H3 cells. In order to explore the inhibitory mechanism of CKS in PCA and mast cell degranulation, we examined the activation of intracellular signaling molecules. CKS suppressed DNP–IgE antibody-induced Syk phosphorylation. Further downstream, CKS also inhibited the phosphorylation of Akt and MAP kinases. Taken together, the in vivo/in vitro anti-allergic effects of CKS suggest possible therapeutic applications for this agent in allergic diseases through the inhibition of inflammatory cytokines and Syk-dependent signaling cascades. |
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Keywords: | Platycodon grandiflorum Anti-allergic activity Mast cells Passive cutaneous anaphylaxis Syk NF-κB |
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