Transfection of a T-cell line with neo increases dexamethasone cytotoxicity |
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Authors: | F de Cuevillas H Lachman |
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Affiliation: | Department of Medicine, Albert Einstein College of Medicine, Bronx, NY 10461. |
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Abstract: | Glucocorticoids (GC) are important therapeutic agents used in the treatment of lymphoid malignancies. GC-mediated cytotoxicity is preceded by the activation of a lysis mechanism induced by DNA nucleosomal cleavage. We have found that transfection of a relatively GC-resistant human T-cell leukemia line with the neo gene, and subsequent selection in geneticin (G418), is associated with enhanced GC-mediated DNA cleavage and cytotoxicity. The conversion of a GC-resistant clone to cells that are GC-sensitive may have implications for experiments involving G418 selection, as well as therapeutic implications in the development of certain types of GC-resistance. |
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