针刺对兔泪液分泌及泪腺血管活性肠肽浓度的影响

目的:观察针刺对兔泪液分泌的影响,以及兔泪腺中神经肽血管活性肠肽(Vasoactive IntestinalPolypeptide,VIP)浓度的变化,从而探讨针刺刺激兔泪液分泌增加的物质基础。方法:以纯种新西兰大白兔为对象进行针刺治疗,取单侧“太阳”“攒竹”“丝竹空”穴,留针20 min,每周3次,共治疗10次。对针刺前后的schirmer试验Ⅰ(shirmer testⅠ,S1T)值进行盲态评分,比较针刺前后评分变化差异,同时用放射免疫法检测针刺前后泪腺VIP浓度的变化。结果:S1T值针刺后即刻[(17.89±3.06)mm/5 min]比针刺前[(10.44±1.81)mm/5 min]明显增高,差异有显著性统计学意义(P<0.001);针刺结束后3 d[(16.89±4.46)mm/5 min]比针刺前明显增高,差异有显著性统计学意义(P<0.01)。说明针刺刺激泪液(主要是水液性)分泌明显增加,且针刺刺激泪液分泌增加有持续性。同时针刺后的泪腺组织VIP浓度[(109.75±22.32)pg/100μg组织]明显高于针刺前[(62.71±20.12)pg/100μg组织],有显著性统计学差异(P<0.001)。结论:针刺能增加泪腺组织中VIP浓度,VIP直接与分泌细胞之间的相互作用刺激了腺体分泌泪液(主要是水液性),而且针刺刺激泪液分泌的作用持久。

Effects of Acupuncture on Tear Secretion and the Concentration of Vasoactive Intestinal Polypeptide in the Lacrimal Gland in Rabbits

Objective: To observe the effect of acupuncture on tear secretion and the concentration of vasoactive intestinal polypeptide(VIP) in the lacrimal gland tissue in the rabbit.Methods: A total of 16 female New Zealand rabbits were randomized into control and acupuncture groups with 8 cases in each group."Taiyang"(EX-HN 5),"Cuanzhu"(BL 2) and "Sizhukong"(TE 23) on the right side were punctured with filiform needles and stimulated for a while with hand by lifting,thrusting and twirling the needle repeatedly.The needles were retained for 20 min and the treatment was conducted 3 times a week with 10 sessions being a therapeutic course.The Schirmer 1 test(S1T,reflecting the basic secretion function) was used to check the secretion function of tear before,immediately and 3 days after acupuncture treatment.The lacrimal gland tissue was sampled and homogenated for assaying the concentrations of VIP before and after acupuncture treatment with radioimmunoassay.Results: Compared with pre-acupuncture the S1T value of acupuncture group increased notably immediately and 3 days after acupuncture [((17.89±3.06) mm/5 min),((16.89±4.46) mm/5 min) vs((10.44±1.81) mm/5 min),(P<0.01)],suggesting an obvious increase in the secretion of tear after acupuncture stimulation.Radioimmunoassay showed that compared with control group the average concentration of VIP in normal lacrimal gland tissue in acupuncture group was significantly higher [((109.75±22.32) pg)/(100 μg) vs((62.71±20.12) pg/100 μg),(P<0.001)].Conclusion: Acupuncture can increase the secretion of tear and raise the concentration of VIP in lacrimal gland tissue,suggesting a direct interaction between VIP and tear secretion from the lacrimal gland.Besides,the influence of acupuncture on the secretion of tear has a long-term aftereffect.