静脉注射免疫球蛋白对新生儿脐血单个核细胞活化增殖的影响

目的探讨静脉注射免疫球蛋白（ＩＶＩＧ）对新生儿单个核细胞活化增殖的影响。方法运用光学显微镜及３Ｈ－ＴｄＲ掺入法观察ＩＶＩＧ对脐血单个核细胞（ＣＢＭＣ）形态学的影响及对ＣＢＭＣ增殖的调节。结果ＩＶＩＧ使ＣＢＭＣ的母细胞化减少，细胞代谢的速度减慢。ＩＶＩＧ使ＣＢＭＣ的３Ｈ－ＴｄＲ掺入率下降更加明显地反映了ＩＶＩＧ对ＣＢＭＣ增殖的抑制。ＩＬ－２或ＩＬ－２联合ＩＬ－４可以拮抗ＩＶＩＧ对ＣＢＭＣ增殖的抑制。结论ＩＶＩＧ并非直接抑制ＣＢＭＣ的活化或干预由ＩＬ－２与ＩＬ－２Ｒ相互作用所介导的增殖反应。ＩＶＩＧ可能通过抑制细胞因子的产生而阻碍ＣＢＭＣ的增殖反应

Modulation of intravenous immunoglobulin on the proliferation in neonatal mononuclear cell

Objective To determine the probable regulatory effects of intravenous immunoglobulin(IVIG) on the proliferation of neonatal mononuclear cells. Method To observe the influence of IVIG on the cord blood mononuclear cells (CBMC) proliferation with both microscopy and the 3 H TdR incorporation test. Results Both cell metabolism and blast transformation of CBMC diminished significantly in the IVIG exposed cells in comparison with the controls. This was further confirmed by 3 H TdR incorporation rates which reflected the inhibitory effect of IVIG on the CBMC proliferation more clearly. Addition of either rIL 2 or the combination of rIL 2 and rIL 4 could antagonise the IVIG induced inhibition. Conclusions Since IVIG did not appear to interfere with either the activation process or IL 2 mediated DNA synthesis and IL 2 receptor interaction of CBMCs, IVIG may inhibit CBMC proliferation, indirectly, through its interference with the production of cytokines