转双反义SBE基因大米的亚慢性毒性实验

目的观察转双反义SBE基因大米是否具有潜在的毒副作用。方法初断乳Wistar大鼠按性别、体重随机分为5组,分别喂饲高剂量亲本大米饲料(A组)、高剂量转基因大米饲料(B组)、中剂量转基因大米饲料(C组)、低剂量转基因大米饲料(D组)和AIN-93G正常对照饲料(E组)。所有动物自由进食饮水,观察大鼠体重、进食量、血常规、血生化、脏器重量、骨密度以及脏器的病理学检查。结果实验中期,B组雌性大鼠的单核细胞百分比显著低于E组(P0.05),谷草转氨酶活性显著高于E组(P0.05)。C组雌性大鼠的谷丙转氨酶活性显著高于A组和E组(P0.05)。实验末期,B组雌性大鼠的谷丙转氨酶和谷草转氨酶活性高于E组(P0.05)。B组雄性大鼠红细胞压积和尿素氮显著低于亲A组(P0.05),E组单核细胞百分比显著高于(P0.05)。B组雌性大鼠大脑的脏器系数显著大于A组(P0.05),B、C、D组雌性大鼠的肾脏脏器系数显著小于E组(P0.05)。实验大鼠各组之间血脂、血钙和骨密度均无统计学差异,内脏病理检查未发现明显异常。结论现有实验结果不能证实该转基因大米对大鼠有亚慢性毒性作用。

Subchronic toxicity test of genetically modified rice with double antisense starch-branching enzyme gene

Objective To observe the sub-chronic toxic effects of the genetically modified rice with double antisense SBE gene.Methods Based on gender and weight,weanling Wistar rats were randomly sorted into five groups: non-genetically modified rice group(group A),genetically modified rice group (group B),half genetically modified rice group( group C),quarter genetically modified rice group( group D) and AIN-93G normal diet group (group E).Indicators were the followings: body weight,food consumption,blood routine,blood biochemical test, organ weight,bone density and pathological examination of organs.Results At the middle of the experiment,the percentage of monocyte of female group B was less than that of group E (P < 0.05).AST activity of female group B was higher than that of group E (P < 0.05).ALT activity of female group C was higher than that of groups A and E (P < 0.05).At the end of the experiment,AST and ALT activities of female group B were higher than those of group E (P < 0.05).Hematocrit (HCT) and blood urea nitrogen ( BUN) level of male group B were less than those of group A (P < 0.05),and monocyte was higher than that of group E ( P < 0.05).The brain index of female group B was higher than group A ( P < 0.05) and the kidney index of group E were higher than those of groups B,C and D (P < 0.05).There were no significant difference of blood lipids,calcium and bone mineral density among all groups (P > 0.05),and no notable abnormity in the pathological examination of main organs ( P > 0.05).Conclusion There were no enough evidence to confirm the sub-chronic toxicity of genetically modified rice on rats.