化学发光法测定甲胎蛋白的临床应用

目的探讨化学发光法测定甲胎蛋白(AFP)的临床应用。方法采用化学发光法测定正常人243例、原发性肝癌患者76例、其它肝病患者165例、非肝癌的癌症患者173例、肝癌术后24例血清中AFP的含量,同时与放射免疫法测定比较。结果以AFP<20ng/ml为正常值,AFP测定的阳性率为:正常人0.8%、原发性肝癌88.1%、其它肝病7.3%、非肝癌的癌症13.87%、肝癌术后41.67%(复发)。与放射免疫法测定比较:相关性R=0.942。结论化学发光法测定AFP结果可靠,能满足临床需要,对于肝癌的诊断和复发有诊断价值。

Detection of Serum AFP with Chemiluminescentassay

Objective To explore the clinical value of chemiluminescence assay on detecting the serum AFP(alpha-fetoprotein). Methods The serum AFP of the patient were measured by chemiluminescentassay and radioimmunoassay respectively: including 243 healthy men, 76 patients with primary hepatic carcinoma, 165 patients with other hepatic diseases, 173 patients with other cancer and 24 patients with primary hepatic carcinoma who underwent operation. Correlation analysis was carried out between the two methods. Results We looked AFP<20ng/ml as normal value. The serum AFP positive rate :healthy men 0.8%, patients with primary hepatic carcinoma 88.1%,patients with other hepatic diseases 7.3%, patients with other cancer 13.87%, patients with hepatic carcinoma who underwent operation 41.67% (recurrence). Distinct correlation was found between chemiluminescence assay and radioimmunological method(R= 0.942). Conclusion The chemiluminescence assay for detection of serum AFP is reliable in clinic and is of great value in diagnosing hepatic carcinoma or its recurrence.