氩氦刀治疗恶性肿瘤细胞免疫功能变化的临床分析

目的探讨恶性肿瘤患者经氩氦刀治疗前后T细胞亚群的变化。方法选取2005年6月-2006年12月的患者，均经病理诊断为Ⅲ期或Ⅳ期的晚期恶性肿瘤，105例患者瘤灶共计109个。病例选择标准：患者预期生存期大于3个月，实验期间均未接受氩氦刀以外的其他治疗。氩氦刀治疗前1周及治疗后1-2周收集标本，做T细胞亚群分析，外周血T细胞亚群CD3^＋、CD4^＋、CD8^＋、CD4^＋／CD8^＋采用流式细胞术检测。结果术后3-12个月复查CT示肿瘤明显缩小，部分患者肿瘤消失。恶性肿瘤患者在氩氦刀治疗后外周血CD3^＋、CD4^＋、CD4^＋／CD8^＋比治疗前明显升高（P〈0．05）。结论恶性肿瘤患者经氩氦刀治疗后可以增强细胞免疫功能，提高抗肿瘤能力。

Clinical analysis of cellular immunity function in malignant tumor treated by cryosurgery

Objective To investigate the T cell subsets changes of patients with malignant tumors treated by Cryosurgery. Methods 109 tumors were collected from 105 patients who have been diagnosed as pathological stage Ⅲ or Ⅳ period advanced cancer and the expected survival time of patients were more than three months since June 2005 to December 2006. Samples were collected one week before and after 1 -2 weeks treatment with Cryosurgery. T - cell subsets of peripheral blood CD3^＋,CD4^＋ , CD8^＋, CD4^＋/CD8^＋ were analyzed using flow cytometry without other treatment. Results After 3 - 12 months of Cryosurgery treatments the tumor was getting reduced significantly on CT and tumor was found disappered in some patients＇s. It was also found that the peripheral blood CD3^＋ , CD4^＋ , CD4^＋/CD8^＋ from malignant tumors patients treated with Cryosurgery were significant increased （P 〈 0. 05 ）compared to that of before treatment. Conclusion Treatment of Cryosurgery can enhance the cellular immune functions and improve the anti - tumor capacity of patients with malignant tumors.