新生儿科产ESBLs大肠埃希菌的基因型研究

目的分析我院新生儿病房分离的大肠埃希菌产ESBLs基因型，为临床合理用药提供依据。方法ESBLs表型筛选、确证试验采用K—B法，采用PCR方法对ESBLs基因型进行检测。结果90株产ESBLs大肠埃希菌进行PCR引物扩增，TEM型引物扩增阳性79株，占87．8％；SHV型引物扩增阳性76株，占84．4％；CTX—M型引物扩增阳性90株，占100％；OXA型引物扩增阳性7株，占7．8％。而72株同时产TEM型、SHV型CTX—M型3种酶。结论在本院新生儿科分离的大肠埃希菌中流行ESBLs的基因型主要是CTX—M型，且同时存在产3种酶，应引起临床重视。

Genotype analysisi of ESBLs-producing Escherichia coli isolated from neonatal unit

Objective To investigate the genotype of ESBLs producing Escherichia colt isolated from neonatal unit. Methods K-B method was preformed to screen the ESBLs phaenotype, and the TEM, SHV, CTX-M, OXA genes were detected by PCR. Results Among 90 ESBLs-producing strains, 79 isolates were TEM positive, 76 isolates were SHV positive, 90 isolates were CTX-M positive, 7 isolates were OXA positive. The gene of TEM, SHV, CTX-M（or OXA） were found co-exitent in 72 strains. Conclusions The main genotype of ESBLs producing Escherichia colt isolated from neonatal unit is blaCTX-M. Most of strains carry 3 ESBLs gene.