A semi-quantitative direct contact assay using fluorescein diacetate and L929 mouse fibroblasts in monolayer culture |
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Authors: | Jennifer L. Burkey Rita A. Brenden |
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Affiliation: | (1) Calgon Vestal Laboratories, P.O. Box 147, 63166 Saint Louis, Missouri |
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Abstract: | Summary A semi-quantitative procedure is described for measuring cell viability after short-term exposure to a test substance using a monolayer culture. Test substances are placed in direct contact with cell monolayers for various time intervals. The substances are removed and the monolayers are incubated in the presence of fluorescein diacetate. Monolayers are viewed under a fluorescent microscope and the percentage of fluorescing (viable) cells is estimated. The method is suitable for examining cytotoxic effects at short times of exposure and for discriminating between test substances that give similar, low toxicity endpoints in standard 24-h assays. |
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Keywords: | in vitro fluorescent dyes cell culture cytotoxicity L929 |
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