| 人乳头瘤病毒18型L2表位的定位 |
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| 引用本文： | 于修平 赵蔚明. 人乳头瘤病毒18型L2表位的定位[J]. 中华实验和临床病毒学杂志, 1995, 9(2): 148-150 |
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| 作者姓名： | 于修平 赵蔚明 |
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| 作者单位： | 山东医科大学微生物学教研室 |
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| 摘 要： | 人乳头瘤病毒１８例的晚期开放读码框架２编码的蛋白具有型特异性。质粒Ｐ１８Ｌ２００１含有ＰＨＶ１８２ＯＲＦ的全序列，经诱导表达产生的融合蛋白能与ＨＰＶ１８感染的患者血清呈阳性免疫反应。采用Ｈｅｎｉｋｏｆｆ改良的单方向缺失核酸链方法，制备了一组从３＇端至５＇端逐步缩小目的ＤＮＡ分子量的质粒，然后表达一系列从Ｃ端至Ｎ端逐步缩小的融合蛋白。用这一系列融合蛋白与相应血清作Ｗｅｓｔｅｒｎｂｌｏｔ实验，最小的阳
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| 关 键 词： | 乳头状瘤病毒 抗原 定位 人乳头瘤病毒 |
Mapping the Location of Human Papillomavirus L2 Open Reading Frame Encoded for Immunoreactive Epitope |
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| Yu Xiuping, Zhao Weiming, Dai Jingyi,et al. Mapping the Location of Human Papillomavirus L2 Open Reading Frame Encoded for Immunoreactive Epitope[J]. Chinese journal of experimental and clinical virology, 1995, 9(2): 148-150 |
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| Authors: | Yu Xiuping Zhao Weiming Dai Jingyi et al |
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| Abstract: | The plasmid p18L2 001 (vector:pATH1,insert:HPV18 4340-5799) was used to map the immunoreactive epitope of HPV18 L2 ORF. Transformed HB101 could express fusion protein,which reacted with sera of patient infected with HPV18.Unidirectional 3'to 5' deletion was made by the insert of p18L2001 using a combination of exonuclease III and nuclease sion proteins with associated in western blot assays.4769 The corresponding peptides were VLDITPSSTSVSISTTNFTNPA FSDPSIIEVPQTGE. |
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| Keywords: | Human papillomavirus type 18 L2 open reading frame Epitope |
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