miR-21 Down-Regulation Suppresses Cell Growth, Invasion and Induces Cell Apoptosis by Targeting FASL, TIMP3, and RECK Genes in Esophageal Carcinoma |
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| Authors: | Na Wang Chao-qi Zhang Jia-huan He Xiao-fei Duan Yuan-yuan Wang Xiang Ji Wen-qiao Zang Min Li Yun-yun Ma Tao Wang Guo-qiang Zhao |
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| Institution: | 1. College of Basic Medical Sciences, Zhengzhou University, Zhengzhou, 450001, China
2. Department of Hemato-Tumor, The First Affiliated Hospital of Henan University of TCM, Zhengzhou, 450000, China
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| Abstract: | Background miR-21 is overexpressed in esophageal squamous cell carcinoma (ESCC) and is thought to be correlated with the development of the cancer. The target gene of miR-21 including FASL, TIMP3 and RECK is revealed by researchers. miR-21 may be involved in the tumorgenesis of ESCC by targeting FASL, TIMP3 and RECK. Aims The purpose of this study was to explore the mechanism of miR-21 in the development of ESCC. Methods miR-21 expression in ESCC and the matched non-malignant adjacent tissues (NMATs) was examined by qRT-PCR. Cell growth, cell apoptosis and cell invasion ability of EC9706 and EC-1 cells was examined after the cells were transfected with miR-21 inhibitor. The potential target genes of miR-21 including FASL, TIMP3 and RECK were examined by western blot and Luciferase reporter assay. Results miR-21 expression was increased significantly in ESCC tissues compared with NMAT. miR-21 down-regulation inhibits cell growth, cell invasion and induces cells to apoptosis. FASL, TIMP3 and RECK are direct targets of miR-21. Conclusions miR-21 down-regulation inhibits cell growth, invasion and induces cells to apoptosis by targeting FASL, TIMP3 and RECK genes. |
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