| 荧光原位杂交与免疫组化对乳腺癌HER-2检测的对比研究 |
| |
| 引用本文： | 董海新,张仁亚,刘启龙,赵元明,孙卓祥,郭焕,赵广章,陈涛. 荧光原位杂交与免疫组化对乳腺癌HER-2检测的对比研究[J]. 济宁医学院学报, 2009, 32(4): 246-249 |
| |
| 作者姓名： | 董海新 张仁亚 刘启龙 赵元明 孙卓祥 郭焕 赵广章 陈涛 |
| |
| 作者单位： | 济宁医学院附属医院,山东,济宁,272029 |
| |
| 基金项目： | 卫生部科研基金课题资助项目 |
| |
| 摘 要： | 目的 探讨荧光原位杂交技术(FISH)检测石蜡标本乳腺癌HER-2基因和免疫组化法(IHC)检测HER-2蛋白表达的结果. 方法 采用荧光原位杂交技术(FISH)和免疫组化技术(IHC)分别检测203例乳腺癌患者手术切除标本的HER-2基因和HER-2蛋白表达,对两种方法检测结果进行对比分析.结果 203例HER-2 IHC结果阳性110例(54%),阴性93例(46%);FISH结果阳性60例(阳性率29%),阴性143例(71%).93例HER-2蛋白为阴性的患者中,HER-2基因表达阴性的87例,符合率93.6%(87/93);58例HER-2蛋白为+的患者中,HER-2基因表达为阳性的11例,符合率为19.0%(11/58);29例HER-2蛋白为++的患者中,HER-2基因表达为阳性的22例,符合率75.9%(22/29);23例HER-2蛋白为的患者中,HER-2基因表达为阳性的21例,两者符合率为91.3%(21/23).结论 对于IHC法初筛为阴性(-)或强阳性(+++)的患者两种方法检测的符合率(≥91.3%)较高,可选择性的进行FISH实验,尤其对于50岁以上年龄组的患者符合率(≥97.7%)更高,而对于IHC法初筛为阳性(+～++) 的患者应再进行FISH实验以确定HER-2基因的状态.
|
| 关 键 词： | 乳腺癌 荧光原位杂交技术 免疫组化法 人类表皮生长因子受体2 |
The comparative study between fluorescence in situ hybridization and immunohistochemistry in detection of breast cancer HER-2 |
| |
| DONG Hai-xin,ZHANG Ren-ya,LIU Qi-long,et al. The comparative study between fluorescence in situ hybridization and immunohistochemistry in detection of breast cancer HER-2[J]. Jounal of Jinning Medical College, 2009, 32(4): 246-249 |
| |
| Authors: | DONG Hai-xin ZHANG Ren-ya LIU Qi-long et al |
| |
| Affiliation: | The Affiliated Hospital of JIning Medical University;Jining 272029;China |
| |
| Abstract: | Objective:To investigate the correlation between the expression of Her-2 gene observed using the fluorescence in situ hybridization(FISH) and the expression of Her-2 protein observed using immunohistochemistry(IHC) in patients of breast cancer.Methods 203 cases of breast cancer were detected by IHC technique and FISH technique respectively.Results In 203 cases of breast cancer,HER-2 was expressed in 54%(110/203) cases,while HER-2 was expressed in 29%(60/203) cases.In the 93 cases that the expression of HER-... |
| |
| Keywords: | Breast carcinoma Fluorescence in situ hybridization Immunohistochemistry Human epidermal growth factor receptor 2 Comparative Study |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
