O(6)-methylguanine DNA methyltransferase activity in diabetic patients |
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Authors: | Akçay Tülay Dinçer Yildiz Celebi Nilgün Ilkova Hasan |
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Affiliation: | Department of Biochemistry Cerrahpa?a Medical School, Istanbul University, Istanbul, Turkey. tulakcay@yahoo.com |
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Abstract: | In the present study, we evaluated O(6)-methylguanine-DNA methyltransferase (MGMT) activity in diabetic patients. The study was performed on 27 patients with Type 1 diabetes, and 42 with Type 2 diabetes. Patients with complications were excluded from the study. 36 non-diabetic volunteers, non-smokers who do not consume alcoholic beverage, were chosen from the medical staff as control subjects. MGMT activity was measured by the transfer of radiolabeled methyl groups from a prepared methylguanine-DNA substrate to the enzyme fraction of leukocyte extract. Leukocyte MGMT activity was significantly reduced in both Type 1 and Type 2 diabetes patients as compared with control subjects (P<0.001). The present study demonstrates decreased MGMT activity in leukocytes from patients with Type 1 and Type 2 diabetes. |
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