医院感染鲍曼不动杆菌分子流行病学研究

目的 分析某医院不同科室来源的鲍曼不动杆菌菌株的同源性，了解鲍曼不动杆菌医院感染流行情况。方法 应用重复片段引物聚合酶链反应（PCR）的基因分型方法，对53株医院感染鲍曼不动杆菌进行基因分型。结果 53株鲍曼不动杆菌分为28个基因型，其中C型15株，分别分离自重症中心、心胸外科的重症监护病房（ICU）、烧伤整形外科、干部病房心内科、创伤手外科、呼吸内科、泌尿内科、儿科ICU、放疗科和血液科；E型和N型各4株，J型3株，O型、Q型和S型各2株，其余均为1株。从同一患者同一部位的不同时期分离出了不同基因型菌株。结论 该院鲍曼不动杆菌以C型菌株为主要的流行株；同一患者存在不同基因型鲍曼不动杆菌的感染。

Molecular epidemiological study of Acinetobacter baumannii nosocomial infection

Objective To study genotypes of Acinetobacter baumannii from different departments in a hospital,and evaluate the prevalence of Acinetobacter baumannii nosocomial infection(NI).Methods Fifty-three strains isolated from patients with NI were typed by genomic DNA by using the enterbacter repetitive intergenic consensus PCR(ERIC-PCR) method.Results Fifty-three strains of Acinetobacter baumannii were divided into 28 genetypes by ERIC-PCR,15 strains were type C.The strains of type C mainly isolated from patients in intensive care unit,coronary care unit,departments of burn and plastic ward,cardiology ward,orthopedic and traumatology surgery,respiratory,urinary,pediatric intensive care unit,radiotherapy and hematology departments,type E,N,J,O,Q and S were 4,4,3,2,2,2 strains,the other types was only 1 strain.Acinetobacter baumannii with different genotypes was isolated from the same site of the same patient during different hospitalization period.Conclusion Type C strains were the main epidemic strains,the same patients were infected with Acinetobacter baumannii of different genotgpes.