小鼠乳腺癌动物模型中调节性T细胞的动态检测及其实验意义

【摘要】 目的 分析小鼠乳腺癌实验动物模型中调节性T细胞(Tregs)的变化，并探讨其在肿瘤发生中的意义。 方法 建立小鼠乳腺癌动物模型。在肿瘤接种后1周、3周和6周3个不同时间点，流式细胞术（FACS）检测CD4+CD25+ 和CD4+ FOXP3+调节性T细胞在脾脏、引流淋巴结和肿瘤组织中所占的CD4+T细胞细胞的比例， CD4+T细胞中CD25+ FOXP3+双阳的表达情况，肿瘤组织中CD4+T细胞占肿瘤浸润T淋巴细胞（CD3+T）的比例。结果 与正常对照组比较，在小鼠乳腺癌动物模型中，脾脏和淋巴结中CD4+ T细胞占淋巴细胞的比例在荷瘤1周、3周和6周逐渐下降（P0. 05）。 结论 调节性T细胞在恶性肿瘤模型中表达增加，在肿瘤的发生发展中发挥重要作用。

Dynamic Detection of Regulatory T Cells in Murine Mammary Carcinoma Model and Its Significance

【Abstract】 Objective To analyze the dynamic change of regulatory T cells in experimental murine mammary carcinoma model，and to investigate their effect on tumor progress. Methods Mouse mammary carcinoma models were established. The percentages of CD4+CD25+ and CD4+ FOXP3+regulatory T cells in the CD4+T cells in tumor tissue, tumor draining lymph node and spleen were measured by FACS at 3 different time points after tumor challenge. The expression of CD25+FOXP3 in CD4+ T cells was analyzed, and the expression of CD4+ T cells in T cells （CD3+）of tumor tissue. Results Compared with the normal control group, In mouse mammary carcinoma models, The percentages of CD4+ T cells to lymphocytes in lymph node and spleen were decreased in the late stage （P0. 05）. Conclusion Regulatory T cells were significantly increased in malignant tumor model, and closely related to the development of tumor.