阿魏酸对血管内皮细胞生长因子诱导的血管平滑肌细胞迁移的影响

目的研究单体阿魏酸对血管平滑肌细胞(vascular smooth muscle cells,VSMCs)迁移的影响,并探讨相关机制。方法体外培养VSMCs,在血管内皮细胞生长因子(vascular endothelial growth factor,VEGF)诱导条件下,用单体阿魏酸进行干预,划痕实验、侵袭实验检测对VSMCs迁移的影响;PT-PCR检测对基质金属蛋白酶(matrix metal loproleinase,MMP)-2、MMP-9mRNA表达的影响;Western blot法检测对金属蛋白酶组织抑制剂(tissue inhibitor of metalloproteinase,TIMP)-1、TIMP-2蛋白表达的影响。结果 (1)在VEGF诱导条件下,阿魏酸102、103ng/mL可抑制VSMCs的迁移;(2)阿魏酸102、103ng/mL可下调VEGF诱导的VSMCs MMP-9mRNA的表达;(3)阿魏酸102、103ng/mL均可上调VEGF诱导的VSMCs TIMP-2蛋白的表达。结论阿魏酸102、103ng/mL均可抑制VEGF诱导的VSMCs迁移,阿魏酸可通过抑制VEGF诱导的VSMCs MMP-9的表达,促进VEGF诱导的VSMCs TIMP-2的表达起到抑制VEGF诱导的VSMCs迁移的作用。

Study on the Effects of Ferulic Acid on the Vascular Smooth Muscle Cell Migration in vitro

Objective To investigate the effects of ferulic acid on the migration of vascular smooth muscle cells(VSMCs) and its correlated mechanisms.Methods VSMCs were in vitro cultured.Under the vascular endothelial growth factor(VEGF) induced conditions,the VSMCs migration were detected using the scratch test and the invasion assay after intervened by ferulic acid.The effects on the mRNA expressions of matrix metalloproteinase,(MMP)-2 and MMP-9 were detected using RT-PCR.The effects on the protein expressions of the tissue inhibitor of metalloproteinase-1(TIMP-1) and TIMP-2 were detected using Western blot.Results(1) Ferulic acid(102 ng/mL and 103 ng/mL) could inhibit VEGF-induced VSMCs migration.(2) Ferulic acid(102 ng/mL and 103 ng/mL) could down-regulate the VEGF-induced VSMCs migration by inhibiting MMP-9 mRNA expression.(3) Ferulic acid(102 ng/mL and 103 ng/mL) up-regulated VEGF-induced VSMCs TIMP-2 protein expressions.Conclusion Ferulic acid(102 ng/mL and 103 ng/mL) could inhibit VEGF-induced VSMCs migration by inhibiting the MMP-9 mRNA expression,and increasing the TIMP-2 protein expression.