BMSCs“归巢”在AMI后心肌重塑中的作用及活血化瘀药的干预研究

目的]通过研究祛瘀生新中药对骨髓间充质干细胞(BMSCs)"归巢"的影响,探讨活血化瘀法对急性心肌梗死(AMI)后心肌重塑的效应机制。方法]清洁级Wistar大鼠80只随机分为5组,分别为正常组、模型组、假手术组、中药组、西药组,每组16只。中药组大鼠术前3 d给药,1次/d。7 d后,检测外周血BMSCs含量,分析各组非梗死区心肌胶原含量,硝基四氮唑蓝(N-BT)染色评价梗死面积,并观察心肌组织苏木-伊红(H-E)染色结果及免疫组化检测心肌c-kit细胞数。结果]药物组较模型组c-kit细胞数均明显增多,中药组与西药组相比较差异无统计学意义(P>0.05)。并能减轻心肌组织的病理损伤及降低心肌胶原含量和梗死面积。结论]活血化瘀法可能通过对BMSCs"归巢"的动员作用,从而有效的抑制AMI后心肌重塑。

Effect of placing on home of BMSCs in myocardial remodeling after AMI and effect of activating blood circulation and removing stasis

Objective] To explore the effective mechanism of activating blood circulation and removing stasis in myocardial remodeling after AMI through researching the effect of removing stasis and promoting tissue regeneration to placing on home of BMSCs. Methods] The 50 rats were randomly divided into five groups: blank control group, myocardial infarction group, sham-operated group, Chinese medicine group, Western medicine group. There were 16 rats in each group. Medicine with effect of removing blood stasis and promoting regeneration was given to treatment group for 3 days before operation, once a day. The rats were sacrificed 7 days later. The peripheral blood BMSCs were detected, the myocardial collagen content of the non-infarction zone was analyzed and the N-BT staining results in colon and immunohistochemical staining myocardial c-kit cell number was observed. Results] The number of myocardial c-kit cell was higher in model-group than sham group. Significant difference was not found between Western medicine and Chinese medicine group (P>0.05). The Chinese medicine could reduce pathological damage of myocardial tissue, decrease the content of myocardial collagen and infact size. Conclusion] Removing blood stasis and promoting regeneration has remarkable inhibitory effect on myocardial remodeling after myocardial infarction and may be through the mobilization function on placing on home of BMSCs.