芒果叶中芒果苷含量的测定

目的]建立芒果叶中芒果苷含量的测定方法;测定不同时期芒果叶中芒果苷的含量。方法]采用高效液相色谱法(HPLC),Cosmosil ODS柱分离,流动相:乙腈-1%冰醋酸溶液(15∶85)为流动相,流速1.0 mL/min,柱温25℃,检测波长254 nm。从海南采集不同时期的芒果叶,测定其中芒果苷含量。结果]芒果苷在0.069 5~2.224 0μg范围内进样量与峰面积呈良好的线性关系,平均回收率96.77%,RSD=1.19%。结论]所建立的芒果苷含量测定方法灵敏度高,准确度高,重现性好。

Determination of Mangiferin content in mango tree leaves

Objective] To investigation the effect of different compatibility of Radix Ginseng and Radix Aconiti Lateralis Preparata on toxicity in mice. Methods] In this study two methods using uniform design and fixed dose of Radix Aconiti praeparata compatibility with Radix Ginseng were used to observe the influence of different proportion in compatibility of Radix Ginseng and Radix Aconiti Lateralis Preparata on acute toxicity in mice. Results] The study of uniform design showed that mice mortality was increased with the dose increasing of Radix Aconiti Lateralis Preparata and with the proportion of Radix Ginseng increased the toxicitiy-decreasing effect had a increasing tendency. The research of the toxicity-attenuated role with the fixed LD₅₀ dose of Radix Aconiti Praeparata and different proportion of Radix Ginseng showed that toxicity- decreasing effect in this compatibility of Radix Ginseng and Radix Aconiti Praeparata increased with the increasing dose of Radix Ginseng in a certain range, especially the dose of Radix Ginseng was more than or equal to the dose of Radix Aconiti Lateralis Preparata. Conclusion] The cutoff point of the effect of attenuation was at a compatibility between Radix Ginseng and Radix Aconiti Lateralis Preparata in 1:1, which can provide some references in he usage of drugs in the clinical practice.