加减温经汤对寒凝血瘀模型大鼠卵巢氧化损伤的影响

目的观察加减温经汤对寒凝血瘀模型大鼠卵巢氧化损伤的影响,探讨加减温经汤治疗寒凝血瘀型妇科疾病的作用机制。方法将SD雌性大鼠随机分为正常组、模型组、治疗组,将模型组和治疗组大鼠置于0～1℃冰水中造成寒凝血瘀大鼠模型;同时治疗组给予加减温经汤灌胃2周。择动情间期断头处死大鼠,检测血清雌二醇(E2)、孕酮(P)、睾酮(T);取双侧卵巢组织,冰盘操作,匀浆后检测总胆红素(TBIL)、超氧化物歧化酶(SOD)、丙二醛(MDA)和总抗氧化能力(T-AOC)。结果与正常组比较,模型组大鼠血清E2、P、T及卵巢TBIL、SOD、T-AOC均明显降低,MDA升高,其差异均有统计学意义(P<0.05或P<0.01)。经加减温经汤治疗后,与模型组比较,大鼠血清E2、P、T及卵巢TBIL、SOD、T-AOC均升高,MDA降低,差异均有统计学意义(P<0.05或P<0.01),除血清T和卵巢MDA,其他值均恢复至正常水平(均P>0.05)。结论加减温经汤可通过改善卵巢的氧化损伤状态,恢复卵巢功能,治疗寒凝血瘀型妇科疾病。

Effects of Modified Wenjing Decoction on the Ovarian Oxidative Damage of Cold Coagulation Blood Stasis Model Rats

Objective To observe the effects of Modified Wenjing Decoction(MWJD) on the ovarian oxidative damage of cold coagulation blood stasis(CCBS) model rats,thus consummating the action mechanisms of MWJD for treating gynecopathy of CCBS syndrome.Methods Female SD rats were randomly divided into the normal group,the model group,and the treatment group.Rats in the model group and the treatment group were placed in the ice water(0-1 ℃) to establish the rat model of CCBS syndrome.Meanwhile,MWJD was given by gastrogavage to rats in the treatment group for 2 weeks.Rats were decapitated during the oestrous cycle.The serum levels of estradiol(E2),progestone(P),testosterone(T) were detected.The bilateral ovarian tissues were taken out and operated on ice.The total bilirubin(TBIL),superoxide dismutase(SOD),malondialdehyde(MDA),total antioxygen capacity(T-AOC) were detected after homogenate.Results Compared with the normal group,serum levels of E2,P,T,and ovarian levels of TBIL,SOD,T-AOC all obviously decreased in the model group,and the ovarian MDA level increased,showing statistical difference(P<0.05,P<0.01).After treated by MWJD,when compared with the model group,serum levels of E2,P,T,and ovarian levels of TBIL,SOD,T-AOC increased,the ovarian MDA level decreased,showing statistical difference(P<0.05,P<0.01).All indices except serum T level and the ovarian MDA content restored to the normal levels(all P>0.05).Conclusions The ovarian functions are abnormal in CCBS model rats.MWJD could restore the ovarian functions by improving the ovarian oxidative damage state,thus treating gynecopathy of CCBS syndrome.