养精种玉汤对多囊卵巢综合征大鼠模型卵巢颗粒细胞分泌功能的影响

目的观察养精种玉汤对母体高雄激素环境建立的子代实验性多囊卵巢综合征(PCOS)模型大鼠血清雌二醇(E2)、睾酮(T)、17-羟孕酮(17-OHP)水平及卵巢内促卵泡激素受体(FSHR)、胰岛素样生长因子1(IGF-1)、类固醇激素急性调节蛋白(StAR)mRNA表达的影响。方法 PCOS大鼠50只,分为对照组(正常大鼠)、模型组、中药低剂量、中剂量、高剂量组,每组10只,运用放射免疫法测定血清激素水平的变化;HE染色法观察卵巢形态学的改变;RT-PCR法检测卵巢FSHR、IGF-1、StAR mRNA的表达。结果与对照组比较,模型组血清T、17-OHP显著升高(P<0.01),E2显著降低(P<0.01),卵巢组织FSHR、IGF-1、StAR mRNA的表达显著降低(P<0.01);与模型组比较,中药高剂量组T显著降低(P<0.01)、17-OHP降低(P<0.05)、E2显著升高(P<0.01),中药低剂量、中剂量、高剂量组FSHR、IGF-1、StAR mRNA的表达均有所上升,尤其是高剂量组的升高最为明显(P<0.01)。结论养精种玉汤可改善PCOS大鼠的高雄激素血症,并能促进卵巢FSHR、IGF-1、StAR mRNA的表达,改善卵巢的功能,促进卵泡发育。

Effects of Yangjing Zhongyu Decoction on the Secretion of Ovarian Granule Cells in Polycystic Ovarian Syndrome Rat Model

Objective To observe the effects of Yangjing Zhongyu Decoction(YJZYD) on the serum estradiol(E2),testosterone(T),17-hydroxyprogesterone(17-OHP),ovarian follicle stimulating hormone receptor(FSHR),insulin-like growth factor Ⅰ(IGF-1),steroid hormone acute regulator protein(StAR) mRNA expressions in female rats.Methods Fifty PCOS rats were equally divided into 5 groups,i.e.,the control group(C,normal PNA rats),the model group(M),the low dose YJZYD group(Y1),the medium dose YJZYD group(Y2),and the high dose YJZYD group(Y3),10 in each.The levels of serum hormones were detected using radioimmunoassay.The morphological changes of the ovary were observed using HE method.The expressions of FSHR,IGF-1,and StAR mRNA were detected using RT PCR.Results Compared with Group C,serum T and 17-OHP significantly increased(P<0.01),E2 significantly decreased(P<0.01),the expressions of FSHR,IGF-1,and StAR mRNA significantly decreased in Group M(P<0.01).Compared with Group M,the serum T level significantly decreased(P<0.01),17-OHP decreased(P<0.05),and E2 significantly increased in Group Y3(P<0.01).The expressions of FSHR,IGF-1,and StAR mRNA increased in Group Y1,Y2,and Y3.The increase was most obvious in Group Y3(P<0.01).Conclusions YJZYD could lower the hyperandrogenemia of PCOS rats.It also could increase the ovarian expressions of FSHR,IGF-1,and StAR mRNA,improve the ovarian functions,and promote the follicular development.