保护性免疫大鼠感染华支睾吸虫后吡喹酮化疗效果

目的 ]研究吡喹酮与宿主保护性免疫对华支睾吸虫感染的协同作用。 方法 ]用粗成虫抗原 (AWAg)和排泄分泌抗原 (ESAg)免疫大鼠或感染 2 0条华支睾吸虫囊蚴获得保护性免疫 ,用 5 0个华支睾吸虫囊蚴感染大鼠 ,用亚致死量吡喹酮(5 0 mg/ kg)治疗 ,以回收虫体数观察疗效。统计学上显著性差异用 PC- SAS系统的 ANOVA和 Nparlway Kruskal- Wallis检验法。 结果 ]1在对照组 ,ESAg免疫接种组和 AWAg免疫接种组吡喹酮杀幼虫效果明显高于成虫 ,3组均为 P<0 .0 1。2感染后的减虫率 ,与对照组比较 ,ESAg免疫接种组 (35 .6 % ,P<0 .0 1)和华支睾吸虫囊蚴感染组 (97.5 % ,P<0 .0 1)较高 ,而在 AWAg免疫接种组 (2 3.4% )较低。吡喹酮的疗效在 ESAg免疫接种组显著增高。 结论 ]吡喹酮对华支睾吸虫的疗效在大鼠具有获得性免疫的情况下相协性的提高。

CHEMOTHERAPEUTIC EFFICACY OF PRAZIQUANTEL IN RATS WITH PROTECTIVE IMMUNITY TO CLONORCHIS SINENSIS INFECTION

Objective] To study the synergic effect of praziquantel(PZQ) and host acquired immunity on Clonorchis sinensis. Methods] Acquired immunity to C.sinensis was induced by immunization with crude adult worm antigen (AW Ag) and excretory\|secretory antigen (ES Ag) or infection with C.sinensis metacercariae. The effect was assessed by the worm reduction rate compared with the control groups after challenge infection with 50 metacercariae and treated orally with a subcurative dose of praziquantel (50 mg/kg). Significant test was performed by analysis of variance (ANOVA) and Npar1 way Kruskal\|Wallis test. All calculations were performed by PC\|SAS system. Results] 1. PZQ was more effective against C.sinensis larvae than against adult worms in the control (P<0\^001), ES Ag (P<0\^01) or crude AW Ag immunization group (P<0\^001). 2. As compared with the control, the worm reduction rate after challenge infection was significantly higher (P<0\^001) in ES Ag immunized group(35\^6%) and metacercaria infection group(97\^5%) and less in crude AW Ag group(23\^4%). The PZQ efficacy was significantly enhanced in ES Ag immunized group. Conclusion] The efficacy of PZQ against C.sinensis could be synergically enhanced in rats by inducing host acquired immunity.