银杏叶提取物对大鼠肺纤维化初期肺结缔组织生长因子表达的影响

目的：　研究银杏叶提取物（GbE）对博莱霉素(BLM)性肺纤维化初期大鼠肺内结缔组织生长因子(CTGF)表达的影响。方法： 气管内滴注BLM, 用Western blotting法检测肺组织CTGF含量；用氯胺-T法测定肺组织羟脯氨酸含量；用比色法检测血浆丙二醛(MDA)含量。结果： 注BLM后14 d肺组织CTGF含量和出肺血MDA含量， BLM+NS组均分别高于NS组(P<0.05; P<0.01)。注BLM后30 d肺组织羟脯氨酸含量和出肺血MDA含量，BLM+NS组均分别高于NS组(Both P<0.01)。GbE处理可显著缓解上述变化。结论： GbE缓解BLM性肺纤维化初期肺内CTGF上调; GbE的抗氧化损伤作用可能是其缓解CTGF增多的机制之一。

Effect of Ginkgo biloba extract on the expression of connective tissue growth factor in the initiating stage of fibrosis in lungs of rats

AIM: To study the effect of Ginkgo biloba extract (GbE) on the expression of connective tissue growth factor (CTGF) in the initiating stage of pulmonary fibrosis of rats after administration of bleomycin (BLM).METHODS: The expression of CTGF in lungs was detected by Western blotting. The content of hydroxyproline was assayed by the method of chloramines T. The content of malondialdehyde (MDA) in plasma was investigated by colorimetry.RESULTS: On day 14 after administration of BLM, the contents of CTGF in lungs and MDA in plasma in BLM NS group were higher than those in NS group, respectively (P<0.05; P<0.01). On day 30 after BLM, the contents of hydroxyproline in lungs and MDA in plasma in BLM NS group were higher than those in NS group, respectively (both P<0.01). Treatment with GbE ameliorated the above changes induced by BLM. CONCLUSION: GbE ameliorates the up-regulation of CTGF in the initial stage of fibrosis in lungs of rats after administration of BLM. GbE prevents the hyperoxidative injury in lungs of rats after BLM, which might be one of mechanisms underling the effect of GbE on CTGF.