MTT法用于实体肿瘤细胞化疗药敏测定的初步研究

用溴化二甲噻唑二苯四氮唑（ＭＴＴ）方法对２６例实体肿瘤细胞行化疗药敏测定。结果表明，不同肿瘤细胞对同一化疗药物，同一肿瘤细胞对不同化疗药物的敏感性各不相同。准确取材、避免污染、合理送检方式以及尽早实验是化疗药敏测定成功的关键。当药敏结果用于肿瘤的个体化治疗时，可选择临床上常用的药物进行试验，并根据结果选对肿瘤抑制率高的前３种药物为相对敏感药物。

STUDY ON THE USE OF MTT ASSAY FOR THE CHEMOSENSITIVITY TESTS OF STEREOTUMOUR CELLS

The chemosensitivity tests of 26 cases stereotumor cells were studied by the MTT assay method.The result showed that 1.MTT assay method was a good one of tumour cell chemosensitivity test; 2.not only the suppressing patterns of one cell line to different chemotherapentic agents, but also different sensitivity of tumour cells to each drug; 3.accurate sampling, avoiding pollution, correct delivery and experiment were very important for finishing chemosensitivity test;4.common drugs should be selected and the first 3 kinds of drugs to suppress tumour cell should be made as relative sensitive drugs when the result of chemosensitivity test was used in individual treatment.