衰老大鼠睾丸生精细胞增殖凋亡及相关因素的变化

目的:观察衰老大鼠睾丸生精细胞增殖、凋亡及相关因素的变化。方法:D-半乳糖连续腹腔注射制作亚急性衰老的大鼠模型。SP免疫组织化学法观察睾丸生精细胞衰老过程中增殖细胞核抗原(PCNA)和端粒酶的表达,TUNEL法观察生精细胞的凋亡,原位杂交、Western印迹分析观察生精细胞p53基因的表达。结果:与正常大鼠相比,衰老大鼠睾丸PCNA阳性细胞率显著下降,端粒酶阳性细胞数明显减少,凋亡管数和凋亡阳性细胞率显著升高,p53 mRNA阳性细胞率和p53蛋白含量均有所升高。结论:衰老大鼠睾丸生精细胞增殖能力下降、凋亡增多,睾丸机能减退。

Changes of proliferation, apoptosis and related factors of spermatogenic cells in the testis of aging rats

Objective:To observe the changes of PCNA expression,apoptosis and related factors of spermatogenic cells in the testis of aging rats.Methods:SD rats were used and divided into the normal control group and aging model group at random.The sub-acutely aging model rats were made by injecting D-gal into abdominal cavity continually.SP immunohistochemistry was used to observe the PCNA and telomerase expression,TUNEL to observe the apoptosis, hybridization in situ and Western blotting to observe the p53 gene expression of the spermatogenic cells.Results:Com- pared with normal rats,the PCNA positive cell rate of aging rats decreased,and the number of telomerase positive cells decreased;the apoptotic index increased significantly,and p53 mRNA positive cell rate and p53 protein contents in- creased significantly.Conclusion:The proliferative ability of the spermatogenic cells decreased,apoptosis increased, and the function of testis was reduced in the aging rats.