新生牛促肝细胞生长素对HL-60细胞增生的影响

Effect of hepatocyte growth-promoting substance on proliferation of HL-60 cell line

AIM: To explore the effect of hepatocyte growth-promoting substance(HGS)on the proliferation of HL-60 cell line and its possible mechanism. METHODS: HL-60 cells were cultured with different concentrations of HGS, As2O3, or As2O3 (107 mol/L) plus HGS(40 mg/L). At day 0,1,2 and 3, cell growth was examined by MTT assay and Trypan blue, cell cycle was measured by flow cytometry. Meanwhile, DNA damage and repair was determined by men single-cell gel eiectrophoresis. RESULTS: HGS inhibited the growth of HL-60 cells in a dose-dependent manner at concentrations ranging from 11.5 to 40 mg/L, with 40 mg/L HGS exhibiting the strongest inhibitory effect. HGS(40 mg/L)not only prevented HL-60 cells from entering G1 phase but also inhibited. DNA damage repair. (-HGS and As2O3synergically inhibited the growth of HL-60 cells. CONCLUSION: HGS can inhibit the proliferation of HL-60 cells at a rather low concentration, which may be related to its effect on the control of cell cycle and the inhibition of DNA repair.