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反相高效液相色谱法测定金鸡胶囊中盐酸巴马汀及盐酸小檗碱的含量
引用本文:黄捷,桑彤,覃红萍. 反相高效液相色谱法测定金鸡胶囊中盐酸巴马汀及盐酸小檗碱的含量[J]. 中国药学杂志, 2002, 22(12): 723-724
作者姓名:黄捷  桑彤  覃红萍
作者单位:广西药品检验所,广西南宁530021
摘    要: 目的:建立测定金鸡股囊中盐酸巴马汀及盐酸小檗碱的含量的方法。方法:反相高效液相色谱法,采用ImetsilODS 3 C18柱为分析柱,乙腈-水-十二烷基磺酸钠(470:530:1)为流动相;检测波长为265 nm,柱温40C,流速为1.0 mL·min-1。结 果:盐酸巴马汀在4.368~52.416 mg·L-1范围内线性关系良好(r=0.9999),平均回收率为98.97% ;盐酸小檗碱在4.532~54.384mg·-1范围内线性关系良好(r=0.9999),平均回收率为98.98%。结论:该法简便,快速,重现性好,适用于金鸡胶囊 中盐酸巴马汀及盐酸小檗碱的定量分析。

关 键 词:金鸡胶囊  盐酸巴马汀  盐酸小檗碱  反相高效液相色谱法
收稿时间:2002-01-08;

Determination of palmatine hydrochloride and berberine hydrochloride in Jinji capsules by RP-HPLC
HUANG Jie,SANG Tong,QIN Hong-Pin,. Determination of palmatine hydrochloride and berberine hydrochloride in Jinji capsules by RP-HPLC[J]. Chinese Pharmaceutical Journal, 2002, 22(12): 723-724
Authors:HUANG Jie  SANG Tong  QIN Hong-Pin  
Affiliation:Guangxi Province Institutial for Drug Control, Guangxi Nanning 530021
Abstract:OBJECTIVE To develop a method for determination of berberine hydrochloride and palmatine hydrochloride in Jinji capsules. METHODS An HPLC method was carried out using an Inertsil ODS-3 column and a mobile phase consisting of acetonitrile-water-sodium laurylsulfonate (470:530:1 g) ,The ultraviolet detection was at 265 nm. Flow rate 1.0 mL·min-1. RESULTS Average recovery of berberine hydrochloride and palmatine hydrochloride were 98.97% and 98.98%, respectively. CONCLUSIONS The standard curve was linear over the range of 4.368~52.416 mg·L-1 for palmatine, and 4.532~54. 384 mg·L-1 for barberine hydrochloride.
Keywords:Jinji capsules')"   href="  #"  >Jinji capsules  berberine hydrochloride  palmatine hydrochloride  PR-HPLC
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