高效液相色谱法测定通脉降糖颗粒中盐酸小檗碱的含量

 目的：测定通脉降糖颗粒中盐酸小檗碱的含量。方法：高效液相色谱法。Spherigel C₁₈柱，乙腈-水（55∶35）为流动相，每1000 ml流动相中含磷酸二氢钾3.4 g,十二烷基硫酸钠1.7 g，流速1 ml·min^-1，检测波长345 nm。结果：通脉降糖颗粒中盐酸小檗碱与多种杂质能有效分离。盐酸小檗碱进样量在0.0265～0.2120 μg范围内线性关系良好，γ=0.9998。平均回收率为101.6% (n=5,RSD=1.8%)。结论：本方法简便、快速、准确。

Determination of berberine hydrochloride in Tongmai Jiangtang granules by HPLC

OBJECTIVE To determine the content of berberine hydrochloride in Tongmai Jiangtang granules.METHODS HPLC was used. Chromatographic conditions included Spherigel C₁₈(250 mm×4.6 mm)column, the mobile phase was acetonitrile-water (55∶35),containing potassium dihydrogen phosphate 3.4 g and sodium dodecyl sulfate 1.7 g per 1000ml. The UV detection wavelength was at 345 nm. RESULTS A satisfactory seperation between berberine hydrochloride and impurities was obtained. The calibration curves was linear in the range of 0.0265～0.2120 μg for berberine hydrochloride (γ=0.9998). The average recovery was 101.6%(n=5,RSD=1.8%). CONCLUSIONS This method is simple, fast and accurative.