高效液相色谱法测定能量合剂中辅酶A的含量

 目的：建立能量合剂中辅酶A含量测定的HPLC方法。方法：采用RP-C₁₈(4.6 mm×200 mm,5 μm)色谱柱，以磷酸盐缓冲液（pH6.5）-甲醇（95∶5）为流动相，流速1.0 ml·min^-1，检测波长259 nm，在室温下对能量合剂中的辅酶A进行含量测定。结果：辅酶A在4～20 IU·ml^-1的范围内，峰面积与效价浓度呈良好线性（r=0.9999）。回收率为100.9%，RSD为1.8%(n=9)。结论：该方法简单，快速，结果准确可靠，可作为能量合剂中辅酶A的含量测定方法。

HPLC determination of coenzyme A in Nengliang Heji for injection

OBJECTIVE To establish an HPLC method for the determination coenzyme A in Nengliangheji for injection. METHODS The column was RP-C₁₈(4.6 mm×200 mm，5 μm)，with pH6.5 phosphate buffer-methanol (95∶5) as mobile phase, The detection wavelength was 259nm at a flow rate of 1.0 ml·min^-1.RESULTS The regression line was liner over the concentration range of 4～20 u·ml^-1(r=0.9999). The recovery percentage of the method was 100.9%, with RSD=1.8%(n=9). CONCLUSIONS The method is simple, rapid and accrete.