离子对高效液相色谱法测定复方甲硝唑片中甲硝唑和维生素B6含量

 目的：建立同时测定复方甲硝唑片中甲硝唑和维生素B₆含量的离子对HPLC法。方法：采用ODS色谱柱,磷酸二氢钾、庚烷磺酸钠溶液（取磷酸二氢钾13.6 g与庚烷磺酸钠1.0 g,加水溶解并稀释成1000 ml）-甲醇-三乙胺（85∶15∶0.4）,用磷酸调pH至2.8为流动相;检测波长290 nm,峰面积外标法。结果：甲硝唑和维生素B₆线性范围分别为100～300 μg·ml^-1 （r=0.9999）,5～50 μ·ml^-1（r=0.9999）;平均回收率分别为100.2% (RSD=0.93%),100.4% （RSD=0.44%）,n=6。结论：离子对HPLC法测定复方甲硝唑片中甲硝唑和维生素B₆含量,方法准确,操作简便,回收率高。

Detertmination of metronidazole and vitamin B6 in compound metronidazole tablets using ion-pair HPLC

OBJECTIVE To establish an ion-pair HPLC method for determination of metronidazole and vitamin B₆ in compound metronidazole tablets.METHODS Column was used. The mobile phase was 0.1 mol·L^-1 KH₂PO₄-methanol-triethylamine (85∶15∶0.4, adjust pH to 2.8 with phosphate acid), and the detective wavelength was 290 nm. RESULTS The linear ranges of metronidazole and vitamin B6 were 100～300 μg·ml^-1(r=0.9999) and 5～50 μg·ml^-1 (r=0.9999), respectively; The average recovery were 100.2%(RSD=0.93%) and 100.4%(RSD=0.44%),n=6.CONCLUSIONS The method is accurate and simple with high recovery.