Effects of MK-801 and Phenytoin on Flurothyl-Induced Seizures During Development |
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| Authors: | Libor Velí &# ek&dagger ,Jana Velí &# ková ,Yael Ptachewich,Shlomo Shinnar&Dagger ,Solomon L. Moshé &dagger &Dagger |
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| Affiliation: | Departments of Neurology, Laboratory of Developmental Epilepsy and Epilepsy Management Center, Montefiore Medical Center, Albert Einstein College of Medicine, Bronx, New York, U.S.A.;Departments of Neuroscience, Laboratory of Developmental Epilepsy and Epilepsy Management Center, Montefiore Medical Center, Albert Einstein College of Medicine, Bronx, New York, U.S.A.;Departments of Pediatrics, Laboratory of Developmental Epilepsy and Epilepsy Management Center, Montefiore Medical Center, Albert Einstein College of Medicine, Bronx, New York, U.S.A. |
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| Abstract: | Summary We determined the effects of the N-methyl-Daspartate (NMDA) receptor blocker MK-801 (0.05, 0.1, and 0.5 mg/kg intraperitoneally, i.p.) and phenytoin (PHT, 5, 10, and 20 mg/kg i.p.) on flurothyl-induced clonic and tonic-clonic seizures in 9-, 1 5, 30-, and 60-day-old male rats. Both agents had seizure-, age-, and dose-specific effects. The highest dose of MK-801 was anticonvulsant against clonic flurothyl-induced seizures only in 9- and 60-day-old rats, but suppressed tonic-clonic seizures in all ages. The lowest dose of MK-801 (0.05 mg/kg) produced significant anticonvulsant effects only in 15 day old rats. PHT did not have any effect on clonic seizures throughout development. Both doses of PHT (10 and 20 mg/kg) were anticonvulsant against tonic-clonic seizures in adult rats but not in any other age group. The results indicate that NMDA receptors play an important role in tonic-clonic flurothyl-induced seizures throughout development (especially in 15-day-old rats) and that the anticonvulsant effects of PHT may vary at different stages of brain development. |
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| Keywords: | Flurothyl Convulsions Developmental biology Phenytoin Anticonvulsants MK-801 |
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