| 痹肿消汤干预胶原诱导关节炎模型大鼠关节滑膜组织细胞外信号调节激酶1/2及细胞外信号调节激酶5的表达 |
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| 引用本文： | 徐则林,梁清华,陈疆,熊新贵,郭亚静,肖玉美. 痹肿消汤干预胶原诱导关节炎模型大鼠关节滑膜组织细胞外信号调节激酶1/2及细胞外信号调节激酶5的表达[J]. 中国组织工程研究与临床康复, 2011, 15(37). DOI: 10.3969/j.issn.1673-8225.2011.37.030 |
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| 作者姓名： | 徐则林 梁清华 陈疆 熊新贵 郭亚静 肖玉美 |
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| 作者单位： | 1. 中南大学湘雅医院,中西医结合研究所,湖南省长沙市,410008
2. 中南大学湘雅医院,远程医疗中心,湖南省长沙市,410008 |
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| 基金项目： | 国家教育部高校博士点基金,湖南省科技计划项目,湖南省中医药基金 |
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| 摘 要： | 背景:滑膜细胞信号转导异常是类风湿性关节炎的重要发病机制之一.中药复方痹肿消汤治疗类风湿性关节炎具有较好的临床疗效,但在细胞信号转导水平探讨痹肿消汤治疗类风湿性关节炎的作用机制是十分必要的.目的:观察中药复方痹肿消汤对胶原诱导关节炎模型大鼠滑膜细胞外信号调节激酶1/2,5表达的影响.方法:SD大鼠45只随机数字表法分为对照组、模型组、痹肿消汤组.胶原诱导关节炎模型大鼠,用痹肿消汤药液3.0～ 3.5 mL灌胃,1次/d,连续14 d.检测造模后第14,21,28天大鼠滑膜细胞外信号调节激酶1/2,5的磷酸化水平.结果与结论:造模后,大鼠滑膜细胞外信号调节激酶1/2,5的磷酸化表达随时间而增加(P < 0.05).痹肿消汤治疗后,细胞外信号调节激酶1/2,5磷酸化表达水平则明显下降(P < 0.05).提示痹肿消汤能抑制细胞外信号调节激酶1/2,5蛋白激酶的激活,从而抑制细胞外信号调节激酶1/2,5信号转导通路的活化,这可能是其阻抑类风湿性关节炎滑膜异常增殖、关节骨质侵蚀的重要机制之一.
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| 关 键 词： | 痹肿消汤 胶原诱导性关节炎 细胞外信号调节激酶1/2 细胞外信号调节激酶5 免疫印迹法 滑膜 |
Effects of Bizhongxiao decoction on extracellular signal-regulated kinase 1/2 and 5 expression in the synovial tissue in a rat model of collagen-induced arthritis |
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| Xu Ze-lin,Liang Qing-hua,Chen Jiang,Xiong Xin-gui,Guo Ya-jing,Xiao Yu-mei. Effects of Bizhongxiao decoction on extracellular signal-regulated kinase 1/2 and 5 expression in the synovial tissue in a rat model of collagen-induced arthritis[J]. Journal of Clinical Rehabilitative Tissue Engineering Research, 2011, 15(37). DOI: 10.3969/j.issn.1673-8225.2011.37.030 |
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| Authors: | Xu Ze-lin Liang Qing-hua Chen Jiang Xiong Xin-gui Guo Ya-jing Xiao Yu-mei |
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| Affiliation: | Xu Ze-lin1,Liang Qing-hua1,Chen Jiang2,Xiong Xin-gui1,Guo Ya-jing1,Xiao Yu-mei1 1Institute of Combined Traditional Chinese and Western Medicine,2Remote Medical Center,Xiangya Hospital of Central South University,Changsha 410008,Hunan Province,China |
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| Abstract: | BACKGROUND:Abnormal signal transduction of synovial cells is one of important pathogeneses of rheumatoid arthritis (RA). Chinese herbs Bizhongxiao decoction exhibits satisfactory clinical curative effects in treatment of RA. It is necessary to investigate the mechanism by which Bizhongxiao decoction treats RA at the level of cellular signal transduction. OBJECTIVE:To investigate the effects of Bizhongxiao decoction on extracellular signal-regulated kinase (ERK) 1/2 and ERK 5 expression in the synovial tissu... |
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