| 壮筋续骨汤含药血清体外培养大鼠成骨细胞的增殖 |
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| 引用本文： | 王力,涂宏,杨凤云,孔令山,鲍杰伟,郑甦.壮筋续骨汤含药血清体外培养大鼠成骨细胞的增殖[J].中国组织工程研究与临床康复,2011,15(37). |
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| 作者姓名： | 王力 涂宏 杨凤云 孔令山 鲍杰伟 郑甦 |
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| 作者单位： | 江西中医学院附属医院关节骨科,江西省南昌市,330006 |
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| 摘 要： | 背景:壮筋续骨汤具有促进胫骨骨折愈合的作用.目的:观察壮筋续骨汤含药血清对体外培养大鼠成骨细胞增殖的影响.方法:大鼠灌胃给予壮筋续骨汤后制备含药血清.取第2代生长状况良好出生48 h内的SD大鼠成骨细胞,对照组和壮筋续骨汤组分别加入5%,10%,20%的正常SD大鼠血清和含药血清培养72 h.结果与结论:MTT法检测发现壮筋续骨汤含药血清对成骨细胞的增殖有明显的促进作用,以10%壮筋续骨汤组作用最明显(P < 0.05).流式细胞仪检测各组细胞DNA合成期(S期)的比例,发现壮筋续骨汤组处于S期的细胞比例明显大于对照组 (P < 0.05).说明壮筋续骨汤含药血清能促进成骨细胞DNA的合成,使更多的细胞进入S期,促进体外培养的成骨细胞增殖.
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| 关 键 词： | 壮筋续骨汤 含药血清 成骨细胞 细胞周期 增殖 |
Effects of Zhuangjinxugu decoction-containing serum on the proliferation of rat osteoblasts cultured in vitro |
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| Wang Li,Tu Hong,Yang Feng-yun,Kong Ling-shan,Bao Jie-wei,Zheng Su.Effects of Zhuangjinxugu decoction-containing serum on the proliferation of rat osteoblasts cultured in vitro[J].Journal of Clinical Rehabilitative Tissue Engineering Research,2011,15(37). |
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| Authors: | Wang Li Tu Hong Yang Feng-yun Kong Ling-shan Bao Jie-wei Zheng Su |
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| Institution: | Wang Li,Tu Hong,Yang Feng-yun,Kong Ling-shan,Bao Jie-wei,Zheng Su Department of Orthopedic Joints,Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine,Nanchang 330006,Jiangxi Province,China |
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| Abstract: | BACKGROUND:Zhuangjinxugu decoction has been shown to promote the healing of tibial fracture. OBJECTIVE:To investigate the effects of Zhuangjinxugu decoction-containing serum on proliferation of rat fibroblasts cultured in vitro. METHODS:Rats were intragastrically administered with Zhuangjinxugu decoction to prepare drug-containing serum. Passage 2 well growing SD rat fibroblasts were harvested within 48 hours after rat birth. Rats from the control and Zhuangjinxugu decoction group were intragastrically admi... |
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