| 人骨肉瘤耐阿霉素细胞模型的建立及其生物学特性 |
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| 引用本文： | 陈渝,王大勇,翁政,邓忠良.人骨肉瘤耐阿霉素细胞模型的建立及其生物学特性[J].中国组织工程研究与临床康复,2011,15(37):6913-6918. |
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| 作者姓名： | 陈渝 王大勇 翁政 邓忠良 |
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| 作者单位： | 1. 重庆市第九人民医院骨科,重庆市,400700
2. 重庆医科大学附属第二医院骨科,重庆市,400010 |
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| 基金项目： | 国家自然科学基金,课题名称:microRNA-1和microRNA-133对骨骼肌卫星细胞增殖分化的调控及机制研究 |
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| 摘 要： | 背景:多药耐药是骨肉瘤化疗失败的重要原因,目前其耐药机制不明。目的:诱导建立耐阿霉素的人骨肉瘤细胞株并观察多药耐药蛋白1、多药耐药相关蛋白1和肺耐药蛋白的表达。方法:采用逐步递增阿霉素浓度间歇作用的方法诱导143B/WT细胞株建立143B/阿霉素耐药细胞株。结果与结论:经阿霉素诱导45d建立了143B/阿霉素细胞株,其对阿霉素高度耐药,对顺铂、甲氨蝶呤、异环磷酰胺、长春新碱和紫杉醇亦产生不同程度交叉耐药;流式细胞仪检测显示与143B野生型细胞相比,143B/阿霉素细胞周期中G1和S期所占比例增加,而G2/M期所占比例明显减少;罗丹明外排实验显示,143B/阿霉素细胞药物外排能力显著高于143B/WT细胞(P<0.01);流式细胞仪和激光共聚焦显微镜观察发现,143B/阿霉素细胞阿霉素相关性细胞凋亡率显著低于143B/WT(P<0.01);Western blot检测显示143B/阿霉素细胞多药耐药蛋白1表达水平较143B/WT显著升高(P<0.01),二者多药耐药相关蛋白1和肺耐药蛋白表达差异无显著性意义(P>0.05)。提示143B/阿霉素细胞多药耐药的产生与多药耐药蛋白1表达升高相关。
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| 关 键 词： | 骨肉瘤 阿霉素 多药耐药 多药耐药蛋白1 细胞模型 |
Establishment of adriamycin-resistant human osteosarcoma cell line and research on its biological characteristics |
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| Chen Yu,Wang Da-yong,Weng Zheng,Deng Zhong-liang.Establishment of adriamycin-resistant human osteosarcoma cell line and research on its biological characteristics[J].Journal of Clinical Rehabilitative Tissue Engineering Research,2011,15(37):6913-6918. |
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| Authors: | Chen Yu Wang Da-yong Weng Zheng Deng Zhong-liang |
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| Institution: | Chen Yu1,Wang Da-yong1,Weng Zheng1,Deng Zhong-liang2 1Department of Orthopedics,Ninth People's Hospital of Chongqing,Chongqing 400700,China,2Department of Orthopedics,Second Affiliated Hospital of Chongqing Medical University,Chongqing 40010 |
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| Abstract: | BACKGROUND:Multidrug resistance is a major factor leading to the failure of chemotherapy for human osteosarcoma. However, the precise mechanism remains poorly understood. OBJECTIVE:To establish adriamycin-resistant human osteosarcoma cell line 143B/ADM and to analyze its biological characteristics. METHODS:Increasing concentrations of adriamycin (ADM) were applied for 45 days to establish 143B/ADM resistant strain. The half of inhibiting concentrations (IC50) and resistance indexs (RI) of different antitumo... |
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