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| 重组人促红细胞生成素后处理缺血/再灌注前后骨骼肌诱导型一氧化氮合酶及一氧化氮的变化 | |
| 引用本文： | 张谦,耿喜林,杨同群,冯宝明,李惠萍,史继德,王秉义.重组人促红细胞生成素后处理缺血/再灌注前后骨骼肌诱导型一氧化氮合酶及一氧化氮的变化[J].中国组织工程研究与临床康复,2011,15(37). |
| 作者姓名： | 张谦 耿喜林 杨同群 冯宝明 李惠萍 史继德 王秉义 |
| 作者单位： | 1. 兰州大学第二医院急救中心创伤外科,甘肃省兰州市,730030 2. 甘肃省第三人民医院药剂科,甘肃省兰州市,730020 3. 甘肃省紧急医疗救援中心医教科,甘肃省兰州市,730030 |
| 摘 要： | 背景:促红细胞生成素对包括心脑在内的多种组织器官的缺血/再灌注损伤有保护作用.目的:观察重组人促红细胞生成素后处理对家兔后肢腓肠肌缺血/再灌注前后诱导型一氧化氮合酶、一氧化氮和超微结构的影响.方法:将家兔随机分为空白对照组、模型组和重组人促红细胞生成素组,后2组建立兔左后肢腓肠肌缺血/再灌注实验模型,其中重组人促红细胞生成素组在兔左后肢缺血2 h后静脉注射重组人促红细胞生成素.结果与结论:再灌注后4,12 h,重组人促红细胞生成素组诱导型一氧化氮合酶表达水平及一氧化氮浓度增高幅度较模型组低(P < 0.05).电镜下腓肠肌细胞超微结构显示,模型组内皮细胞膜溶解,肿胀明显,肌纤维内线粒体水肿.重组人促红细胞生成素组肌纤维损伤较轻,肌节内Z线及肌节内各带结构基本正常,大部分线粒体结构正常,显示重组人促红细胞生成素组超微结构损伤程度明显轻于模型组.结果证实,重组人促红细胞生成素后处理可通过抑制诱导型一氧化氮合酶蛋白的表达,减少再灌注后过量一氧化氮生成,改善骨骼肌缺血/再灌注损伤. |
| 关 键 词： | 重组人促红细胞生成素 骨骼肌 缺血/再灌注损伤 诱导型一氧化氮合酶 一氧化氮 超微结构 组织构建 |
Effects of recombinant human erythropoietin postconditioning on inducible nitric oxide synthase and nitric oxide in rabbit hindlimb gastrocnemius before and after ischemia/reperfusion injury |
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| Zhang Qian,Geng Xi-lin,Yang Tong-qun,Feng Bao-ming,Li Hui-ping,Shi ji-de,Wang Bing-yi.Effects of recombinant human erythropoietin postconditioning on inducible nitric oxide synthase and nitric oxide in rabbit hindlimb gastrocnemius before and after ischemia/reperfusion injury[J].Journal of Clinical Rehabilitative Tissue Engineering Research,2011,15(37). | |
| Authors: | Zhang Qian Geng Xi-lin Yang Tong-qun Feng Bao-ming Li Hui-ping Shi ji-de Wang Bing-yi |
| Institution: | Zhang Qian1,Geng Xi-lin1,Yang Tong-qun1,Feng Bao-ming2,Li Hui-ping3,Shi ji-de1,Wang Bing-yi1 1Department of Emergency Medicine,Second Hospital of Lanzhou University,Lanzhou 730030,Gansu Province,China,2Department of Pharmacy,Third People's Hospital of Gansu Province,Lanzhou 730020,3Department of Medical Education,Emergency Medical Rescue Center in Gansu Province |
| Abstract: | BACKGROUND:In recent years, many studies have shown that erythropoietin plays a protective role in many tissues and organs from ischemia/reperfusion injury including the heart and brain. OBJECTIVE: To observe the effects of recombinant human erythropoietin postconditioning on inducible nitric oxide synthase, nitric oxide and ultrastructure in rabbit hindlimb gastrocnemius before and after ischemia/reperfusion injury. METHODS:Adult rabbits were randomly divided into three groups: blank control, model, and re... |
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