K562/NOD-SCID小鼠白血病模型的建立

本研究探讨人CML急性变的白血病细胞在NOD—SCID小鼠体内建立白血病模型的方法并研究其生长特性。首先将K562细胞接种于全身受照射后的裸鼠，待皮下成瘤后取出局部瘤块，选取无坏死的瘤组织制成瘤细胞悬液，再腹腔接种于全身受照射后的NOD—SCID小鼠。结果表明：成功建立全身播散的白血病模型。4周时外周血涂片可见白血病细胞，晚期浸润肝、脾、骨髓等造血器官，白细胞上升到接种前的8—10倍，血涂片中白血病细胞达20％-30％。腹腔局部出现瘤块，多位于腹腔内或大网膜，较少累及其他器官。结论：腹腔接种K562瘤细胞于全身照射后的NOD—SCID小鼠能建成全身播散的白血病模型，该模型较好地反映白血病在体内的演变过程，是进行新药疗效试验、生物导向治疗及基因治疗的理想工具。

Establishment of K562/NOD-SCID Mouse Model with Leukemia

The study was aimed to establish a K562/NOD-SCID leukemia mouse model and to explore its growth characteristics. Nude mice exposed to total body irradiation were inoculated subeutaneouly with K562 cells, then the local K562 tumor was taken out and the tumor tissues nithout necoosis were selected for preparing single cell suspension which was inoculated into irradiated NOD/SCID mice by intraperitoneal injection. The results indicated that the systemic disseminated leukemia model was established successfully by intraparitoneal injection. On the fourth week after inoculation the leukemia cells were found on peripheral blood smear, and the leukemia cell infiltration was observed in liver, spleen and bone marrow. On the brink of death, the count of peripheral blood WBC was 8 - 10 times as much as that before inoculation. The leukemia cells on peripheral blood smear acounted to 20% -30% of the total WBCs on average. The local tumors appeared in the abdominal cavity or on the greater omentum, less were involved in other organs. It is concluded that the established K562/NOD-SCID mouse model with leukemia well imitates the process of leukemia in human body, so it is a good model for the research on the effects of new drugs and target or gene therapy.