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Isolation of human rotavirus in cell cultures |
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| Authors: | K. Sato Y. Inaba T. Shinozaki R. Fujii M. Matumoto |
| Affiliation: | (1) National Institute of Animal Health, Tsukuba, Ibaraki-ken, Japan;(2) Department of Pediatrics, Teikyo University School of Medicine, Itabashi-ku, Tokyo, Japan;(3) Kitasato Institute, Minato-ku, Tokyo, Japan |
| Abstract: | Summary Three cytopathic rotavirus strains were isolated in MA104 cells from faecal specimens of pediatric patients with acute gastroenteritis. Pre-treatment of virus with trypsin and incorporation of a small amount of trypsin in maintenance medium were important for establishment of the strains in these cells. The isolates were antigenically closely related with strain Wa of human rotavirus and had some antigenic relationship with strain Lincoln of bovine rotavirus.With 1 Figure |
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