蚯蚓冻干粉对高血脂症小鼠载脂蛋白AI和载脂蛋白B基因表达的影响

目的研究蚯蚓冻干粉(earthworm lyophilized powder,ELP)的降血脂作用是否与调节载脂蛋白AI(apolipoproteinAI,ApoAI)和载脂蛋白B(apolipoprotein B,ApoB)基因表达有关。方法健康昆明种小鼠60只,随机分为5组,基础饲料组(normal diet control group,NCG)、高脂饲料组(high fatdiet control group,HCG)、高脂饲料+高剂量ELP组(high-dose gavage group,HDG)、高脂饲料+中剂量ELP组(middle-dose gavage group,MDG)、高脂饲料+低剂量ELP组(low-dose gavage group,LDG)。ELP干预10周后,测定各组小鼠血脂水平,RT-PCR测定各组小鼠肝脏ApoAI、ApoB mRNA表达量。结果 ELP明显降低高血脂小鼠血浆TG(totalcholesterol)、TG(triglycerol)和LDL-C(low-density lipoprotein-cholesterol)水平,同时升高HDL-C(high-density lipoprotein-cholesterol)水平。HDG组和MDG组小鼠ApoAI mRNA表达量明显上升,ApoB mRNA表达量明显降低。结论 ELP具有明显的降血脂作用,其降血脂作用可能与其调节载脂蛋白基因表达有关。

Effects of earthworm lyophilized powder on ApoAI and ApoB gene expression in hyperlipidemic mice

Aim To investigate whether the modulating effect of earthworm lyophilized powder(ELP)on serumlipids in experimental hyperlipidemic mice was relevant to the regulation of the ApoAI and ApoB gene expressions in liver.Methods Kunming mice were randomly divided into 5 groups:Normal diet control group(NCG),High fat diet control group(HCG),High-dose gavage group(HDG),Middle-dose gavage group(MDG)and Low-dose gavage group(LDG).After 10 weeks,the mice were killed and the tail blood was taken for the determination of blood lipids,the liver was dropped into liquid nitrogen immediately for the extraction of total RNA,the mRNA expressions of ApoAI and ApoB in mice liver were quantified by RT-PCR.Results ELP lowered the TC,TG,LDL-C levels and increased HDL-C levels of the hyperlipidemic mice remarkably.ELP increased ApoAI gene mRNA expressions significantly in HDG and MDG mice,however,ELP decreased ApoB gene mRNA expressions markedly in the above two groups.Conclusions ELP lowered the blood lipids level of the hyperlipidemic mice significantly,its mechanism might be related to enhancing the expression of ApoAI gene and reducing the expression of ApoB gene in liver.