糖尿病大鼠卵巢组织中细胞色素C、线粒体Ca^2＋变化与灵芝孢子粉的干预

目的：以卵巢组织中细胞色素C、线粒体Ca^2＋和性激素水平为观察指标，观察灵芝孢子粉对2型糖尿病大鼠卵巢组织的保护作用。方法：实验于2006—06／08在佳木斯大学黑龙江省小儿神经康复重点实验室完成。①实验对象：Wistar雌性大鼠50只，随机分为对照组10只，2型糖尿病模型组20只，灵芝孢子粉组20只。②实验方法：大鼠禁食16～18h，自由饮水，模型组及灵芝孢子粉组大鼠经尾静脉一次性按25mg／kg注射2％链脲佐菌素，正常对照组尾静脉注射等量柠檬酸钠-柠檬酸缓冲液。正常饮食喂养2周后，进行糖耐量试验，模型组和灵芝组以糖耐量异常者保留，余去除，并改喂高脂高糖饮食，灵芝孢子粉组另加灵芝孢子粉250mg／（kg·d）]持续10周，实验结束前一天再次做糖耐量试验。③实验评估：实验结束后，各组动物均禁食12h，乙醚吸入浅麻醉，内眦静脉取血待测血清胰岛素。解剖出卵巢，待测线粒体细胞色素C、线粒体Ca^2＋含量。结果：选用Wistar雌性大鼠50只，结果分析数量每组8只。①葡萄糖耐量试验：实验开始及实验12周时，模型组大鼠均表现出糖耐量异常的变化（P〈0．05），1h血糖增高显著（P〈0．05）达到高峰，2h血糖仍较高；对照组大鼠30min血糖达到峰值，2h血糖基本恢复正常。②血清胰岛素和卵巢性激素水平：模型组血清胰岛素明显升高（P〈0．05）。而灵芝孢子粉组与对照组比较差异无显著性。模型组雌二醇含量明显降低（P〈0．05），卵胞刺激素含量、睾酮含量明显升高（P〈0．05），而灵芝孢子粉组与对照组比较差异无显著性。③胞浆细胞色素C、线粒体细胞色素C、线粒体钙变化：模型组卵巢组织胞浆细胞色素C含量明显升高（P〈0．05），模型组卵巢组织线粒体细胞色素C含量明显降低（P〈0．05），模型组卵巢组织线粒体钙含量明

Effect of Ganoderma lucidum spore powder on cytochrome C and mitochondrial Ca^2＋ in the ovaries of diabetic rats

AIM： To explore the effect of Ganoderma lucidum spore powder on the ovaries of diabetic rats by observing the levels of cytochrome C, mitochondrial Ca^2＋ and gonadal hormone. METHODS： The experiment was conducted in the Key Laboratory of Child Neural Rehabilitation of Heilongjiang Province from June to August 2006. （1）Fifty female Wistar rats were randomly divided into control group （n =10）, type 2 diabetes model group （n =20） and Ganoderma group （n =20）. （2）The rats were fasted for 16-18 hours but drank freely. Model group and Ganoderma group were injected 25 mg/kg streptozotocin （STZ, 2%） one time by vena caudalis; Control group was injected matching citrate sodium-citric acid buffer solution. After two weeks of normal diet, carbohydrate tolerance test was carried out. Only the rats of model group and Ganoderma group showed abnormal in carbohydrate tolerance test were remained and feed with high fat and high glucose foods. Besides that, Ganoderma group was added Ganoderma lucidum spore powder （250 mg/kg daily） for 10 weeks. Carbohydrate tolerance test was carried out again before the end of the experiment. （3）The animals were fasted for 12 hours and anesthetized with ether to collect angular vein blood to detect serum insulin. The ovaries were harvested to determine the content of cytochrome C and mitochondrial Ca^2＋. RESULTS： Totally 50 rats were enrolled and finally 8 of each group were involved in the result analysis. （1）In the carbohydrate tolerance test before and 12 weeks of experiment, the tolerance was changed in model rats （P 〈 0.05）, at 1 hour, blood glucose level was significantly increased （P 〈 0.05） and reached the peak; at 2 hours, the blood glucose level was still high. In the model group, the blood glucose level reached the peak at 30 minutes, and restored to normal level at 2 hours. （2）The serum insulin of model group was significantly increased （P 〈 0.05）. There was no obvious difference between Ganoderma group and control gro