缝线诱导角膜新生血管形成：术后核因子κB表达的动态变化

背景：角膜新生血管化不但严重影响视力，也是同种异体角膜移植术后发生排斥反应的高危因素，因此研究角膜新生血管发病机制和能阻止其形成的抑制剂，一直是眼科学的热点和难点。目的：采用缝线法建立大鼠角膜新生血管模型，以地塞米松作为糖皮质激素类角膜新生血管抑制剂，探讨核因子κB在角膜新生血管发生发展中的作用机制。设计：随机对照的实验研究。单位：南方医科大学珠江医院眼科。材料：实验于2005—01／04在解放军第一军医大学珠江医院眼科完成。选用55只出生二三个月雄性健康清洁级Wistar大鼠。兔抗大鼠核因子κB P65多克隆抗体，兔抗大鼠血管内皮生长因子、细胞间粘附分子1多克隆抗体均购自武汉博士德生物工程有限公司。方法：①实验干预：摸球法随机将大鼠分为生理盐水组（n=25）、地塞米松组（n=25）、正常角膜组（n=5）。生理盐水对照组及地塞米松组大鼠采用缝线法建立大鼠角膜新生血管模型后，分别给予生理盐水及地塞米松眼液点右眼，2滴，次，3次／d，至术后18d。正常角膜组无干预措施。②实验评估：术后1，3，7，12，18d对生理盐水对照组及地塞米松组大鼠进行角膜新生血管生长评分，取各组角膜切片后光镜下观察各组角膜组织学变化，并采用免疫组织化学染色检测各组角膜核因子κB与细胞间粘附分子1、血管内皮生长因子的表达。主要观察指标：①角膜新生血管生长评分。②角膜组织学变化。③各组角膜核因子κB与细胞间粘附分子1、血管内皮生长因子的表达。结果：①角膜新生血管生长评分：地塞米松组角膜新生血管受到了明显的抑制，其术后各时间点的角膜新生血管评分均低于生理盐水组（P〈0．05～0．01）。②角膜组织学变化：地塞米松组角膜缝线诱导后新生血管及炎性细胞浸润均较对?

Nylon suture induced corneal neovascularization： Postoperative dynamic changes of nuclear factor-kappa B expression

BACKGROUND：Corneal neovascularization not only seriously affects vision, it is also a high-risk factor for the rejection after allogeneic ceratoplasty, thus it is always a hot issue and in ophthalmology to investigate the pathogenesis of corneal neovascularization and the inhibitors for blocking its formation. OBJECTIVE： To induce model of corneal neovascularization in rats using nylon suture, and investigate the mechanism of nuclear factor-κB （NF-κB） in the occurrence and development of corneal neovascularization using dexamethasone as the glucocorticoid inhibitor for corneal neovascularization. DESIGN ： A randomized controlled trial.SETTING ： Department of Ophthalmology, Zhujiang Hospital of Southern Medical University.MATERIALS ： The experiments were carried out in the Zhujiang Hospital of Southern Medical University from January to April 2005. Fifty-five healthy male Wistar rats of clean degree were used. Rabbit-anti-rat NF-κB P65 monoclonal antibody, rabbit-anti-rat vascular endothelial growth factor （VEGF） and intercellular adhesion molecule-1 （ICAM-1） monoclonal antibodies, and 3,3-diaminobenzidine （DAB） kit were purchased from Wuhan Boster Biological Technology Co.. Ltd. METHODS： （1） Interventions： The rats were randomly divided in the saline control group （n =25）, dexamethasone group （n =25） and normal cornea group （n =5）. Corneal neovascularization using nylon suture was induced in rats in the saline control group and dexamethasone group, and then saline and dexamethasone was dropped to the right eye of the rats respectively, 2 drops for each time, 3 times a day for 18 days. Not any treatment was given to the rats in the normal cornea group. （2） Evaluations： The score of corneal neovascularization was evaluated in the saline control group and dexamethasone group at 1, 3, 7, 12 and 18 days postoperatively. Corneal sections were prepared to observe the histological changes of cornea under light microscope; The expressions of NF-κB, VEGF and ICAM-1 we