金莲花不同花部对荭草素和牡荆素的贡献度研究

目的：建立一种评价不同花部对金莲花主要成分贡献度的综合方法，为金莲花的育种，培养，开发和利用提供依据。方法：重量分析法测定不同花部的质量分数，高效液相色谱法测定不同花部荭草素和牡荆素的含量，采用数学方法计算各花部对金莲花中荭草素和牡荆素的贡献度，并通过Kruskal．WallisH检验和主成分分析对各花部的贡献度进行差异分析。结果：花萼、花冠、花蕊群、花柄和子房对荭草素和牡荆素的贡献度分别为76．99％和71．93％，9．60％和8．33％，9．21％和8．10％，2．17％和6．62％，2．03％和5．02％。结论：不同花部对荭草素和牡荆素的贡献不均等，其中，花萼贡献度最大，并且与其他花部具有极显著差异。

Contribution evaluation of the floral parts to orientin and vitexin concentrations in the flowers of Trollius chinensis

AIM： To provide a comprehensive procedure to evaluate the contribution of the floral parts to the yield of the major components from the flowers of Trollius chinensis, to underlay the selective breeding, cultivation, development, and utilization of the flowers. METHODS： Five floral parts from eleven batches of the flowers of T. chinensis were examined by HPLC analysis for the content of orientin and vitexin, and by gravimetric analysis for their respective mass fraction. The contribution of each floral part was calculated using mathematical methods based on the results of the content and mass fraction. Variance analysis was carried out by Kruskal-Wallis H test and PCA method. RESULTS： The calculated mean contributions of calyx, corolla, stamens and pistils, stalk, and ovary to the yield of both orientin and vitexin were 76.99% and 71.93%, 9.60% and 8.33%, 9.21% and 8.10%, 2.17% and 6.62%, and 2.03% and 5.02%, respectively. CONCLUSION： The floral parts contribute unequally to the yield of orientin and vitexin, and the calyx contributes the highest and makes a significant difference compared with any other part.