大鼠壁细胞K+离子通道特性的研究

目的研究大鼠壁细胞K^＋离子通道的特性，探讨其在生理病理过程中的作用。方法分离大鼠壁细胞，采用膜片钳方法，对大鼠壁细胞K^＋离子通道进行全细胞电生理记录，分析壁细胞K^＋离子通道的基本特性。结果10mM氯化TEA部分阻断壁细胞外向去极化K^＋离子通道电流。10mM乙酸TEA、10mM氯化钡、10mM乙酸钡能使壁细胞外向去极化K^＋离子通道电流增加。结论大鼠壁细胞的全细胞K^＋离子电流具有电压依赖性，兼有外向整流性和内向整流性。

Studies on the property of potassium-ion channels of rat parietal cells

Objective To study the properties of K~ +-ion channel of rat parietal cells, and to approach its effects on physiological and pathological process. Method Parietal cells were isolated. Electrophysiological records of K~ +-ion channels were made through whole cell's patch clamp technique, and the properties of the channels were analyzed. Results 10mM tetraethylammonium chloride may partially block the outwardly depolarized K~ +-ion channel current. While 10mM tetraethylammonium acetate, 10mM barium chloride or 10mM barium acetate may increases the outwardly depolarized K~ +-ion channel current. Conclusion The whole cell K~ +-ion channel current of rat parietal cells showed voltage sensitive. The current-voltage relations of whole cell potassium channels of rat parietal cells are both outwardly and inwardly rectified.