| 不同牙周状况龈下菌斑中齿垢密螺旋体的分布 |
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| 引用本文： | 朱丽芳,郑瑜谦,闫福华. 不同牙周状况龈下菌斑中齿垢密螺旋体的分布[J]. 口腔医学研究, 2010, 26(1): 67-70 |
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| 作者姓名： | 朱丽芳 郑瑜谦 闫福华 |
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| 作者单位： | 福建医科大学附属口腔医院牙周病科,福建,福州,350002 |
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| 基金项目： | 福建省青年人才项目基金(编号:2006F3053) |
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| 摘 要： | 目的：分析慢性牙周炎、牙龈炎患者和牙周健康者龈下菌斑中齿垢密螺旋体的分布情况，探讨该微生物与不同牙周状况的关系。方法：收集12例慢性牙周炎、5例牙龈炎患者和5例牙周健康者，共70个位点的龈下菌斑，采用Taq Man16SrRNA实时荧光定量PCR技术检测齿垢密螺旋体的分布。结果：慢性牙周炎病变位点齿垢密螺旋体检出率是86％，牙龈炎的是86％，牙周健康的是1.0％，3组检出率之间的差异没有统计学意义；微生物的检出量经对数转换后，3组之间及两两比较均有统计学意义。结论：TaqMan实时荧光定量PCR技术检测微生物灵敏度高，检出率高；龈下菌斑中齿垢密螺旋体与牙周状态密切相关。
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| 关 键 词： | 齿垢密螺旋体 牙周 实时荧光定量PCR |
Prevalence of Treponema Denticola in Relation to Periodontal Status. |
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| ZHU Li-fang,ZHENG Yu-qian,YAN Fu-hua. Prevalence of Treponema Denticola in Relation to Periodontal Status.[J]. Journal of Oral Science Research, 2010, 26(1): 67-70 |
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| Authors: | ZHU Li-fang ZHENG Yu-qian YAN Fu-hua |
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| Affiliation: | ZHU Li-fang,ZHENG Yu-qian,YAN Fu-hua. Department of Periodontology,School of Stomatology,Fujian Medical University,Fuzhou 350002 |
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| Abstract: | Objective:To investigate the distribution of T. denticola in chronic periodontitis patients,gingivitis and healthy subjects. Methods:70 subgingival plaque samples were collected from 12 chronic periodontitis patients,5 gingivitis and 5 healthy subjects. T. denticola was detected by 16S rRNA DNA TaqMan real-time fluorescence quantitative PCR. Results:The detection rates of Td in chronic periodontitis was 86%,in gingivitis was 70%,and in periodontal health was 100%,which were not significant different. The qu... |
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| Keywords: | Treponema denticola Periodontal TaqMan real-time PCR |
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