Suppression of lipopolysaccharide-induced of inducible nitric oxide synthase and cyclooxygenase-2 by Sanguis Draconis, a dragon's blood resin, in RAW 264.7 cells |
| |
Authors: | Choy Cheuk-Sing Hu Chien-Ming Chiu Wen-Ta Lam Carlos-Shu Kei Ting Yih Tsai Shin-Han Wang Tzu-Chien |
| |
Affiliation: | Emergency Department of Municipal Wan Fang Hospital, Taipei Medical University, Taipei, Taiwan, ROC. |
| |
Abstract: | Sanguis Draconis (SD) is a kind of dragon's blood resin that is obtained from Daemomorops draco (Palmae). It is used in traditional medicine and has shown anti-inflammatory activity in some diseases. In this study, we examined the effects of Sanguis Dranonis ethanol extract (SDEE) on LPS-induced inflammation using RAW 264.7 cells. Our data indicated that SDEE inhibits LPS-stimulated NO, PGE2, IL-1 beta and TNF-alpha release, and iNOS and COX-2 expression. Furthermore, SDEE suppressed the LPS-induced p65 expression of NF-kappa B, which was associated with the inhibition of I kappa B-alpha degradation. We also found that the expression of HO-1 was significantly increased in RAW 264.7 cells by SDEE. These results suggest among possibilities of anti-inflammation that SDEE inhibits the production of NO and PGE2 by the down-regulation of iNOS and COX-2 gene expression via the suppression of NF-kappaB (p65) activation. SDEE can induce HO-1 over-expression in macrophage cells, which indicates that it may possess antioxidant properties. This result means that SEDD its anti-inflammatory effects in macrophages may be through a novel mechanism that involves the action of HO-1. Thus, SD could provide a potential therapeutic approach for inflammation-associated disorders. |
| |
Keywords: | COX-2, cyclooxygenase-2 eNOS, endothelial nitric oxide synthase HO-1, heme oxgenase-1 IκB-α, inhibitory factor κB-α IL-1β, interlukin-1β iNOS, inducible nitric oxide synthase LPS, lipopolysaccharide NF-κB, nuclear factor-κB NO, nitric oxide nNOS, neuronal nitric oxide synthase PGE2, prostaglandin E2 SD, Sanguis Draconis SDEE, Sanguis Draconis ethanol extract TNF-α, tumor necrosis factor-α |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|