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| 罗格列酮抑制垂体腺瘤GH3细胞及促凋亡作用的研究 | |
| 引用本文： | 董齐,胡志强,伊然,孔宪刚.罗格列酮抑制垂体腺瘤GH3细胞及促凋亡作用的研究[J].中国微侵袭神经外科杂志,2007,12(8):363-366. |
| 作者姓名： | 董齐 胡志强 伊然 孔宪刚 |
| 作者单位： | 1. 哈尔滨医科大学附属第一医院,神经外科,黑龙江,哈尔滨,150001 2. 北京世纪坛医院神经外科,北京,100038 3. 哈尔滨医科大学附属第一医院,内分泌科,黑龙江,哈尔滨,150001 4. 中国农业科学院哈尔滨兽医研究所,黑龙江,哈尔滨,150001 |
| 摘 要： | 目的探讨罗格列酮对GH3细胞增殖的影响及其促细胞凋亡的机制。方法不同浓度罗格列酮作用GH3细胞后,流式细胞仪检测细胞周期和细胞凋亡,Elisa法分析罗格列酮对生长激素合成的影响,Western blot法分析GH3细胞Cyclin D3、bcl-2和bax的变化。结果罗格列酮作用GH3细胞48 h后,以浓度效应关系抑制GH3细胞增殖、并使细胞增殖周期中G0-G1期阻滞,S期和G2-M期百分率降低;Western blot法示罗格列酮作用GH3细胞提高了Cyclin D3和bax的表达,而bcl-2的表达降低。结论罗格列酮促GH3细胞凋亡并抑制GH分泌,可能成为垂体生长激素腺瘤病人新的治疗方法。 |
| 关 键 词： | 垂体肿瘤 生长激素 噻唑烷二酮类 |
| 文章编号： | 1009-122X（2007）08-0363-04 |
| 修稿时间： | 2007-02-12 |
Rosiglitazone induces growth inhibition and apoptosis of pituitary GH3 cells |
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| DONG Qi, HU Zhiqiang, YI Ran, et al.Rosiglitazone induces growth inhibition and apoptosis of pituitary GH3 cells[J].Chinese Journal of Minimally Invasive Neurosurgery,2007,12(8):363-366. | |
| Authors: | DONG Qi HU Zhiqiang YI Ran |
| Institution: | 1. Department of Neurosurgery, 3, Department of Endocrinology, First Hospital Affiliated to Harbin Medical University, Harbin 150001, China; 2. Department ofNeurosurgery, Shijitan Hospital, Beijing 100038, China |
| Abstract: | Objective To explore the effects of rosiglitazone on rat GH-secreting(GH3) cell proliferation and its role in apoptosis.Methods After different concentrations of rosiglitazone acted on GH3 cells,the cell cycle and apoptosis were assessed by FACS analysis.The effects of rosiglitazone on GH synthesis were evaluated by ELISA.Expressions of cyclin D3,bcl-2 and bax were evaluated by Western blotting.Results After treatment of CH3 cells with rosiglitazone for 48 h,the cell proliferation was inhibited in a concentration-dependent manner.G0-G1 cell cycle phase was blocked and cell proportion in the S and G2-M phase decreased significantly.Rosiglitazone increased the expression of cyclin D3 and bax,decreased the expression of bcl-2.Conclusion Rosiglitazone suppress GH secretion as well as apoptosis and has the potential of being used to treat pituitary growth hormone-secreting adenomas |
| Keywords: | pituitary neoplasms growth hormone thiazolidincdiones |
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